Scanning ion conductance microscopy measurement of paracellular channel conductance in tight junctions

Chiao Chen Chen; Yi Zhou; Celeste A. Morris; Jianghui Hou; Lane A. Baker

doi:10.1021/ac303441n

Scanning ion conductance microscopy measurement of paracellular channel conductance in tight junctions

Chiao Chen Chen, Yi Zhou, Celeste A. Morris, Jianghui Hou, Lane A. Baker

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

Elucidation of epithelial transport across transcellular or paracellular pathways promises to advance the present understanding of ion transport and enables regulation of cell junctions critical to the cell and molecular biology of the epithelium. Here, we demonstrate a new instrumental technique, potentiometric scanning ion conductance microscopy (P-SICM), that utilizes a nanoscale pipet to differentiate paracellular and transcellular transport processes at high spatial resolution. The technique is validated for well-defined polymer membranes and then employed to study wild type and claudin-deficient mutants of Madin-Darby Canine Kidney strain II (MDCKII) cells. Paracellular permeabilities conferred by claudin-2 are captured by P-SICM which demonstrates the utility to monitor apparent conductance at subcellular levels.

Original language	English
Pages (from-to)	3621-3628
Number of pages	8
Journal	Analytical Chemistry
Volume	85
Issue number	7
DOIs	https://doi.org/10.1021/ac303441n
Publication status	Published - 2013 Apr 2

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.1021/ac303441n

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abstract = "Elucidation of epithelial transport across transcellular or paracellular pathways promises to advance the present understanding of ion transport and enables regulation of cell junctions critical to the cell and molecular biology of the epithelium. Here, we demonstrate a new instrumental technique, potentiometric scanning ion conductance microscopy (P-SICM), that utilizes a nanoscale pipet to differentiate paracellular and transcellular transport processes at high spatial resolution. The technique is validated for well-defined polymer membranes and then employed to study wild type and claudin-deficient mutants of Madin-Darby Canine Kidney strain II (MDCKII) cells. Paracellular permeabilities conferred by claudin-2 are captured by P-SICM which demonstrates the utility to monitor apparent conductance at subcellular levels.",

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AU - Chen, Chiao Chen

AU - Zhou, Yi

AU - Morris, Celeste A.

AU - Hou, Jianghui

AU - Baker, Lane A.

PY - 2013/4/2

Y1 - 2013/4/2

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AB - Elucidation of epithelial transport across transcellular or paracellular pathways promises to advance the present understanding of ion transport and enables regulation of cell junctions critical to the cell and molecular biology of the epithelium. Here, we demonstrate a new instrumental technique, potentiometric scanning ion conductance microscopy (P-SICM), that utilizes a nanoscale pipet to differentiate paracellular and transcellular transport processes at high spatial resolution. The technique is validated for well-defined polymer membranes and then employed to study wild type and claudin-deficient mutants of Madin-Darby Canine Kidney strain II (MDCKII) cells. Paracellular permeabilities conferred by claudin-2 are captured by P-SICM which demonstrates the utility to monitor apparent conductance at subcellular levels.

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Scanning ion conductance microscopy measurement of paracellular channel conductance in tight junctions

Abstract

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