Screening of high daptomycin-producing strains by combined antibiotics resistance screening method

Zhi Xiang Zhang, Ran Duan, Ke Ju Jing, I-Son Ng, Ying Hua Lu

Research output: Contribution to journalArticle

Abstract

Objective Combined antibiotics resistance screening method was applied to screen daptomycin high-producing strain. Methods Based on the biosynthetic pathway and metabolization mechanism of daptoomycin, combined antibiotics resistance screening method was applied to screen daptomycin high-producing strain from original strain of Streptomyces roseosporus ATCC 11379. Results A mutant strain D1000-S3-2 was obtained. The yield of daptomycin was 59 mg/L in flask by the mutant strain, which increased 63.8%, compared to that of the parental strain. Conclusion It was demonstrated that the method provides a fast and effective way of screening S. roseosporus.

Original languageEnglish
Pages (from-to)202-206
Number of pages5
JournalChinese Journal of Antibiotics
Volume37
Issue number3
Publication statusPublished - 2012 Aug 22

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Daptomycin
Microbial Drug Resistance
Biosynthetic Pathways
Streptomyces

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Zhang, Zhi Xiang ; Duan, Ran ; Jing, Ke Ju ; Ng, I-Son ; Lu, Ying Hua. / Screening of high daptomycin-producing strains by combined antibiotics resistance screening method. In: Chinese Journal of Antibiotics. 2012 ; Vol. 37, No. 3. pp. 202-206.
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Screening of high daptomycin-producing strains by combined antibiotics resistance screening method. / Zhang, Zhi Xiang; Duan, Ran; Jing, Ke Ju; Ng, I-Son; Lu, Ying Hua.

In: Chinese Journal of Antibiotics, Vol. 37, No. 3, 22.08.2012, p. 202-206.

Research output: Contribution to journalArticle

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PY - 2012/8/22

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AB - Objective Combined antibiotics resistance screening method was applied to screen daptomycin high-producing strain. Methods Based on the biosynthetic pathway and metabolization mechanism of daptoomycin, combined antibiotics resistance screening method was applied to screen daptomycin high-producing strain from original strain of Streptomyces roseosporus ATCC 11379. Results A mutant strain D1000-S3-2 was obtained. The yield of daptomycin was 59 mg/L in flask by the mutant strain, which increased 63.8%, compared to that of the parental strain. Conclusion It was demonstrated that the method provides a fast and effective way of screening S. roseosporus.

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