Selective detection of dopamine in urine with electrodes modified by gold nanodendrite and anionic self-assembled monolayer

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Abstract

The determination of dopamine (DA) in urine is a promising technique for non-invasive DA monitoring, but current electrochemical techniques face substantial interference from uric acid (UA). This study applies three-dimensional gold nanodendrite (Au-DT) and self-assembled monolayer (SAM) to modify gold electrodes to improve their sensitivity and specificity to DA and alleviate UA interference. With the optimal ω-mercaptoalkane carboxylic acid chain length, the differential pulse voltammograms of Au-DT deposited electrode coated with 8-mercaptooctanoic acid (MOA) exhibit two voltammetric peaks that represent the oxidation potentials of DA and UA, respectively, with a separation of 260 mV. Amperometric measurements show that the response current of Au-DT/MOA linearly depends on DA in the range of 0.01-5 μM. The sensitivity of the Au-DT/MOA modified electrode is 20-fold that of the MOA-coated electrode. The proposed electrode has a low detection limit of 20 nM for DA at a signal to noise ratio of 3 and resistance to UA interference, showing no response current with UA addition and no obvious decrease in the sensitivity of DA detection in the presence of UA. Moreover, the performances of the developed electrode, including sensitivity, recovery, and reproducibility, are validated for detecting DA in non-diluted human urine, with satisfactory results.

Original languageEnglish
Pages (from-to)179-186
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume181
DOIs
Publication statusPublished - 2013 Mar 25

Fingerprint

dopamine
urine
Self assembled monolayers
uric acid
Gold
Dopamine
Uric Acid
gold
Electrodes
electrodes
Acids
acids
sensitivity
interference
Carboxylic Acids
Chain length
carboxylic acids
Signal to noise ratio
signal to noise ratios
Carboxylic acids

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

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title = "Selective detection of dopamine in urine with electrodes modified by gold nanodendrite and anionic self-assembled monolayer",
abstract = "The determination of dopamine (DA) in urine is a promising technique for non-invasive DA monitoring, but current electrochemical techniques face substantial interference from uric acid (UA). This study applies three-dimensional gold nanodendrite (Au-DT) and self-assembled monolayer (SAM) to modify gold electrodes to improve their sensitivity and specificity to DA and alleviate UA interference. With the optimal ω-mercaptoalkane carboxylic acid chain length, the differential pulse voltammograms of Au-DT deposited electrode coated with 8-mercaptooctanoic acid (MOA) exhibit two voltammetric peaks that represent the oxidation potentials of DA and UA, respectively, with a separation of 260 mV. Amperometric measurements show that the response current of Au-DT/MOA linearly depends on DA in the range of 0.01-5 μM. The sensitivity of the Au-DT/MOA modified electrode is 20-fold that of the MOA-coated electrode. The proposed electrode has a low detection limit of 20 nM for DA at a signal to noise ratio of 3 and resistance to UA interference, showing no response current with UA addition and no obvious decrease in the sensitivity of DA detection in the presence of UA. Moreover, the performances of the developed electrode, including sensitivity, recovery, and reproducibility, are validated for detecting DA in non-diluted human urine, with satisfactory results.",
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AU - Chen, Jia Jin Jason

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AB - The determination of dopamine (DA) in urine is a promising technique for non-invasive DA monitoring, but current electrochemical techniques face substantial interference from uric acid (UA). This study applies three-dimensional gold nanodendrite (Au-DT) and self-assembled monolayer (SAM) to modify gold electrodes to improve their sensitivity and specificity to DA and alleviate UA interference. With the optimal ω-mercaptoalkane carboxylic acid chain length, the differential pulse voltammograms of Au-DT deposited electrode coated with 8-mercaptooctanoic acid (MOA) exhibit two voltammetric peaks that represent the oxidation potentials of DA and UA, respectively, with a separation of 260 mV. Amperometric measurements show that the response current of Au-DT/MOA linearly depends on DA in the range of 0.01-5 μM. The sensitivity of the Au-DT/MOA modified electrode is 20-fold that of the MOA-coated electrode. The proposed electrode has a low detection limit of 20 nM for DA at a signal to noise ratio of 3 and resistance to UA interference, showing no response current with UA addition and no obvious decrease in the sensitivity of DA detection in the presence of UA. Moreover, the performances of the developed electrode, including sensitivity, recovery, and reproducibility, are validated for detecting DA in non-diluted human urine, with satisfactory results.

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