Selective expression of a “correct cloud” of Dscam in crayfish survivors after second exposure to the same pathogen

Tze Hann Ng, Ramya Kumar, Kantamas Apitanyasai, Shu Ting He, Shen Po Chiu, Han Ching Wang

Research output: Contribution to journalArticle

Abstract

Arthropod hypervariable Dscam (Down syndrome cell adhesion molecule) may be involved in adaptive-like immune characteristics, namely immune priming, enabling the host to “learn” and “remember” pathogens previously encountered in arthropods. However, expression of Dscam in immune-primed arthropods after a second challenge has apparently not been confirmed. Herein, working with Dscam of Australian freshwater crayfish (Cherax quadricarinatus, i.e. CqDscam), we further investigated whether immune priming is mediated by “clouds” of appropriate (or “correct”) CqDscam isoforms. In crayfish that survived a first WSSV challenge (immune priming), long-lasting CqDscam expression remained higher after a second WSSV challenge. Selective CqDscam isoforms were also induced after both challenges. Based on pathogen binding assays, these WSSV-induced CqDscam isoforms had a higher WSSV binding ability, perhaps mainly mediated by Ig3-spliced variants. We therefore hypothesized that in these crayfish survivors, an unknown selection process was generating a “correct cloud” of CqDscam against a previously encountered pathogen.

Original languageEnglish
Pages (from-to)430-437
Number of pages8
JournalFish and Shellfish Immunology
Volume92
DOIs
Publication statusPublished - 2019 Sep

Fingerprint

Down syndrome
Cell Adhesion Molecules
Pathogens
crayfish
adhesion
cell adhesion
arthropod
arthropods
Protein Isoforms
pathogen
pathogens
Cherax quadricarinatus
binding capacity
Assays
assay
assays
exposure

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

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abstract = "Arthropod hypervariable Dscam (Down syndrome cell adhesion molecule) may be involved in adaptive-like immune characteristics, namely immune priming, enabling the host to “learn” and “remember” pathogens previously encountered in arthropods. However, expression of Dscam in immune-primed arthropods after a second challenge has apparently not been confirmed. Herein, working with Dscam of Australian freshwater crayfish (Cherax quadricarinatus, i.e. CqDscam), we further investigated whether immune priming is mediated by “clouds” of appropriate (or “correct”) CqDscam isoforms. In crayfish that survived a first WSSV challenge (immune priming), long-lasting CqDscam expression remained higher after a second WSSV challenge. Selective CqDscam isoforms were also induced after both challenges. Based on pathogen binding assays, these WSSV-induced CqDscam isoforms had a higher WSSV binding ability, perhaps mainly mediated by Ig3-spliced variants. We therefore hypothesized that in these crayfish survivors, an unknown selection process was generating a “correct cloud” of CqDscam against a previously encountered pathogen.",
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Selective expression of a “correct cloud” of Dscam in crayfish survivors after second exposure to the same pathogen. / Ng, Tze Hann; Kumar, Ramya; Apitanyasai, Kantamas; He, Shu Ting; Chiu, Shen Po; Wang, Han Ching.

In: Fish and Shellfish Immunology, Vol. 92, 09.2019, p. 430-437.

Research output: Contribution to journalArticle

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