Abstract
Arthropod hypervariable Dscam (Down syndrome cell adhesion molecule) may be involved in adaptive-like immune characteristics, namely immune priming, enabling the host to “learn” and “remember” pathogens previously encountered in arthropods. However, expression of Dscam in immune-primed arthropods after a second challenge has apparently not been confirmed. Herein, working with Dscam of Australian freshwater crayfish (Cherax quadricarinatus, i.e. CqDscam), we further investigated whether immune priming is mediated by “clouds” of appropriate (or “correct”) CqDscam isoforms. In crayfish that survived a first WSSV challenge (immune priming), long-lasting CqDscam expression remained higher after a second WSSV challenge. Selective CqDscam isoforms were also induced after both challenges. Based on pathogen binding assays, these WSSV-induced CqDscam isoforms had a higher WSSV binding ability, perhaps mainly mediated by Ig3-spliced variants. We therefore hypothesized that in these crayfish survivors, an unknown selection process was generating a “correct cloud” of CqDscam against a previously encountered pathogen.
| Original language | English |
|---|---|
| Pages (from-to) | 430-437 |
| Number of pages | 8 |
| Journal | Fish and Shellfish Immunology |
| Volume | 92 |
| DOIs | |
| Publication status | Published - 2019 Sep |
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All Science Journal Classification (ASJC) codes
- Environmental Chemistry
- Aquatic Science
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Selective expression of a “correct cloud” of Dscam in crayfish survivors after second exposure to the same pathogen. / Ng, Tze Hann; Kumar, Ramya; Apitanyasai, Kantamas; He, Shu Ting; Chiu, Shen Po; Wang, Han Ching.
In: Fish and Shellfish Immunology, Vol. 92, 09.2019, p. 430-437.Research output: Contribution to journal › Article
TY - JOUR
T1 - Selective expression of a “correct cloud” of Dscam in crayfish survivors after second exposure to the same pathogen
AU - Ng, Tze Hann
AU - Kumar, Ramya
AU - Apitanyasai, Kantamas
AU - He, Shu Ting
AU - Chiu, Shen Po
AU - Wang, Han Ching
PY - 2019/9
Y1 - 2019/9
N2 - Arthropod hypervariable Dscam (Down syndrome cell adhesion molecule) may be involved in adaptive-like immune characteristics, namely immune priming, enabling the host to “learn” and “remember” pathogens previously encountered in arthropods. However, expression of Dscam in immune-primed arthropods after a second challenge has apparently not been confirmed. Herein, working with Dscam of Australian freshwater crayfish (Cherax quadricarinatus, i.e. CqDscam), we further investigated whether immune priming is mediated by “clouds” of appropriate (or “correct”) CqDscam isoforms. In crayfish that survived a first WSSV challenge (immune priming), long-lasting CqDscam expression remained higher after a second WSSV challenge. Selective CqDscam isoforms were also induced after both challenges. Based on pathogen binding assays, these WSSV-induced CqDscam isoforms had a higher WSSV binding ability, perhaps mainly mediated by Ig3-spliced variants. We therefore hypothesized that in these crayfish survivors, an unknown selection process was generating a “correct cloud” of CqDscam against a previously encountered pathogen.
AB - Arthropod hypervariable Dscam (Down syndrome cell adhesion molecule) may be involved in adaptive-like immune characteristics, namely immune priming, enabling the host to “learn” and “remember” pathogens previously encountered in arthropods. However, expression of Dscam in immune-primed arthropods after a second challenge has apparently not been confirmed. Herein, working with Dscam of Australian freshwater crayfish (Cherax quadricarinatus, i.e. CqDscam), we further investigated whether immune priming is mediated by “clouds” of appropriate (or “correct”) CqDscam isoforms. In crayfish that survived a first WSSV challenge (immune priming), long-lasting CqDscam expression remained higher after a second WSSV challenge. Selective CqDscam isoforms were also induced after both challenges. Based on pathogen binding assays, these WSSV-induced CqDscam isoforms had a higher WSSV binding ability, perhaps mainly mediated by Ig3-spliced variants. We therefore hypothesized that in these crayfish survivors, an unknown selection process was generating a “correct cloud” of CqDscam against a previously encountered pathogen.
UR - http://www.scopus.com/inward/record.url?scp=85067681257&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85067681257&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2019.06.023
DO - 10.1016/j.fsi.2019.06.023
M3 - Article
C2 - 31200075
AN - SCOPUS:85067681257
VL - 92
SP - 430
EP - 437
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
SN - 1050-4648
ER -