Sensing of diseased mitochondria proportion by DEP at the organelle level of intact cells

Pei Yin Chi, Ting Wei Chuang, Tzu Tsai Chu, Chia Tzu Kuo, Yu Ting Wu, Vahid Farmehini, Dar Bin Shieh, Fan Gang Tseng, Yau Huei Wei, Nathan Swami, Chia Fu Chou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To monitor the healthiness of cells, it is very important to develop quick ways to characterize cells with the diseased and normal mitochondria. Thus, we can take measures to prevent potential development of mitochondria-related diseases. Physical study and systemic proof of concept by engineering method to perform quick diagnosis of the normal and diseased mitochondria have not been well achieved yet. In this study, we used insulator dielectrophoresis (i-DEP), by utilizing the intrinsic dielectric response of a cell to an external AC electric field to differentiate cells containing partially diseased and normal mitochondria to test if we can select diseased mitochondria-containing cells but not the normal cells. We found that it is possible to detect the diseased and healthy mitochondria in cells without breaking the cell membrane. Taking advantage of the dielectrophoresis, DEP principle, we may develop a quick diagnostic method to detect diseased mitochondria in living cells.

Original languageEnglish
Title of host publicationMicrofluidics, BioMEMS, and Medical Microsystems XVIII
EditorsBonnie L. Gray, Holger Becker
PublisherSPIE
ISBN (Electronic)9781510632332
DOIs
Publication statusPublished - 2020
EventMicrofluidics, BioMEMS, and Medical Microsystems XVIII 2020 - San Francisco, United States
Duration: 2020 Feb 12020 Feb 4

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11235
ISSN (Print)1605-7422

Conference

ConferenceMicrofluidics, BioMEMS, and Medical Microsystems XVIII 2020
CountryUnited States
CitySan Francisco
Period20-02-0120-02-04

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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