Separation of mitochondrial diseased cells based on organelle-level difference using a dep microfluidic system

Pei Yin Chi, Ting Wei Chuang, Tzu Tsai Chu, Chia Tzu Kuo, Yu Ting Wu, Vahid Farmehini, Dar Bin Shieh, Fan Gang Tseng, Yau Huei Wei, Nathan Swami, Chia Fu Chou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

It is imperative to develop tools to characterize cells with the diseased and normal mitochondria to monitor the healthiness of cells and take preventive measures for potential mitochondrial disease development. Here, we adopt insulator dielectrophoresis (i-DEP), by utilizing the intrinsic dielectric response of a cell to an external AC electric field to differentiate cells, containing partially diseased and normal mitochondria. Taking advantage of dielectrophoresis, DEP, principle, we developed a quick diagnostic method to detect mitochondrial disease in living cells, which is not readily achievable by conventional cell cytometry.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages1372-1373
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 2019 Oct 272019 Oct 31

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Country/TerritorySwitzerland
CityBasel
Period19-10-2719-10-31

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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