Sequencing batch reactor enhances bacterial hydrolysis of starch promoting continuous bio-hydrogen production from starch feedstock

Shing D. Chen, Yung Chung Lo, Kuo Shing Lee, Tian I. Huang, Jo-Shu Chang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Bio-hydrogen production from starch was carried out using a two-stage process combining thermophillic starch hydrolysis and dark H2 fermentation. In the first stage, starch was hydrolyzed by Caldimonas taiwanensis On1 using sequencing batch reactor (SBR). In the second stage, Clostridium butyricum CGS2 was used to produce H2 from hydrolyzed starch via continuous dark hydrogen fermentation. Starch hydrolysis with C. taiwanensis On1 was operated in SBR under pH 7.0 and 55 °C. With a 90% discharge volume, the reducing sugar (RS) production from SBR reactor reached 13.94 g RS/L, while the reducing sugar production rate and starch hydrolysis rate was 0.92 g RS/h/L and 1.86 g starch/h/L, respectively, which are higher than using other discharge volumes. For continuous H2 production with the starch hydrolysate, the highest H2 production rate and yield was 0.52 L/h/L and 13.2 mmol H2/g total sugar, respectively, under a hydraulic retention time (HRT) of 12 h. The best feeding nitrogen source (NH4HCO3) concentration was 2.62 g/L, attaining a good H2 production efficiency along with a low residual ammonia concentration (0.14 g/L), which would be favorable to follow-up photo H2 fermentation while using dark fermentation effluents as the substrate.

Original languageEnglish
Pages (from-to)8549-8557
Number of pages9
JournalInternational Journal of Hydrogen Energy
Volume34
Issue number20
DOIs
Publication statusPublished - 2009 Oct 1

Fingerprint

sequencing
starches
Batch reactors
hydrogen production
Hydrogen production
Starch
Feedstocks
hydrolysis
Hydrolysis
reactors
sugars
fermentation
Sugars
Fermentation
Clostridium
effluents
hydraulics
ammonia
Effluents
Ammonia

All Science Journal Classification (ASJC) codes

  • Energy Engineering and Power Technology
  • Fuel Technology
  • Renewable Energy, Sustainability and the Environment
  • Condensed Matter Physics

Cite this

@article{c4c06baba9424de6881eaceaec429f4b,
title = "Sequencing batch reactor enhances bacterial hydrolysis of starch promoting continuous bio-hydrogen production from starch feedstock",
abstract = "Bio-hydrogen production from starch was carried out using a two-stage process combining thermophillic starch hydrolysis and dark H2 fermentation. In the first stage, starch was hydrolyzed by Caldimonas taiwanensis On1 using sequencing batch reactor (SBR). In the second stage, Clostridium butyricum CGS2 was used to produce H2 from hydrolyzed starch via continuous dark hydrogen fermentation. Starch hydrolysis with C. taiwanensis On1 was operated in SBR under pH 7.0 and 55 °C. With a 90{\%} discharge volume, the reducing sugar (RS) production from SBR reactor reached 13.94 g RS/L, while the reducing sugar production rate and starch hydrolysis rate was 0.92 g RS/h/L and 1.86 g starch/h/L, respectively, which are higher than using other discharge volumes. For continuous H2 production with the starch hydrolysate, the highest H2 production rate and yield was 0.52 L/h/L and 13.2 mmol H2/g total sugar, respectively, under a hydraulic retention time (HRT) of 12 h. The best feeding nitrogen source (NH4HCO3) concentration was 2.62 g/L, attaining a good H2 production efficiency along with a low residual ammonia concentration (0.14 g/L), which would be favorable to follow-up photo H2 fermentation while using dark fermentation effluents as the substrate.",
author = "Chen, {Shing D.} and Lo, {Yung Chung} and Lee, {Kuo Shing} and Huang, {Tian I.} and Jo-Shu Chang",
year = "2009",
month = "10",
day = "1",
doi = "10.1016/j.ijhydene.2009.08.043",
language = "English",
volume = "34",
pages = "8549--8557",
journal = "International Journal of Hydrogen Energy",
issn = "0360-3199",
publisher = "Elsevier Limited",
number = "20",

}

Sequencing batch reactor enhances bacterial hydrolysis of starch promoting continuous bio-hydrogen production from starch feedstock. / Chen, Shing D.; Lo, Yung Chung; Lee, Kuo Shing; Huang, Tian I.; Chang, Jo-Shu.

In: International Journal of Hydrogen Energy, Vol. 34, No. 20, 01.10.2009, p. 8549-8557.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Sequencing batch reactor enhances bacterial hydrolysis of starch promoting continuous bio-hydrogen production from starch feedstock

AU - Chen, Shing D.

AU - Lo, Yung Chung

AU - Lee, Kuo Shing

AU - Huang, Tian I.

AU - Chang, Jo-Shu

PY - 2009/10/1

Y1 - 2009/10/1

N2 - Bio-hydrogen production from starch was carried out using a two-stage process combining thermophillic starch hydrolysis and dark H2 fermentation. In the first stage, starch was hydrolyzed by Caldimonas taiwanensis On1 using sequencing batch reactor (SBR). In the second stage, Clostridium butyricum CGS2 was used to produce H2 from hydrolyzed starch via continuous dark hydrogen fermentation. Starch hydrolysis with C. taiwanensis On1 was operated in SBR under pH 7.0 and 55 °C. With a 90% discharge volume, the reducing sugar (RS) production from SBR reactor reached 13.94 g RS/L, while the reducing sugar production rate and starch hydrolysis rate was 0.92 g RS/h/L and 1.86 g starch/h/L, respectively, which are higher than using other discharge volumes. For continuous H2 production with the starch hydrolysate, the highest H2 production rate and yield was 0.52 L/h/L and 13.2 mmol H2/g total sugar, respectively, under a hydraulic retention time (HRT) of 12 h. The best feeding nitrogen source (NH4HCO3) concentration was 2.62 g/L, attaining a good H2 production efficiency along with a low residual ammonia concentration (0.14 g/L), which would be favorable to follow-up photo H2 fermentation while using dark fermentation effluents as the substrate.

AB - Bio-hydrogen production from starch was carried out using a two-stage process combining thermophillic starch hydrolysis and dark H2 fermentation. In the first stage, starch was hydrolyzed by Caldimonas taiwanensis On1 using sequencing batch reactor (SBR). In the second stage, Clostridium butyricum CGS2 was used to produce H2 from hydrolyzed starch via continuous dark hydrogen fermentation. Starch hydrolysis with C. taiwanensis On1 was operated in SBR under pH 7.0 and 55 °C. With a 90% discharge volume, the reducing sugar (RS) production from SBR reactor reached 13.94 g RS/L, while the reducing sugar production rate and starch hydrolysis rate was 0.92 g RS/h/L and 1.86 g starch/h/L, respectively, which are higher than using other discharge volumes. For continuous H2 production with the starch hydrolysate, the highest H2 production rate and yield was 0.52 L/h/L and 13.2 mmol H2/g total sugar, respectively, under a hydraulic retention time (HRT) of 12 h. The best feeding nitrogen source (NH4HCO3) concentration was 2.62 g/L, attaining a good H2 production efficiency along with a low residual ammonia concentration (0.14 g/L), which would be favorable to follow-up photo H2 fermentation while using dark fermentation effluents as the substrate.

UR - http://www.scopus.com/inward/record.url?scp=70349459607&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349459607&partnerID=8YFLogxK

U2 - 10.1016/j.ijhydene.2009.08.043

DO - 10.1016/j.ijhydene.2009.08.043

M3 - Article

VL - 34

SP - 8549

EP - 8557

JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

SN - 0360-3199

IS - 20

ER -