Sequential dark-photo fermentation and autotrophic microalgal growth for high-yield and CO2-free biohydrogen production

Yung Chung Lo, Chun Yen Chen, Chi Mei Lee, Jo Shu Chang

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)


Dark fermentation, photo fermentation, and autotrophic microalgae cultivation were integrated to establish a high-yield and CO2-free biohydrogen production system by using different feedstock. Among the four carbon sources examined, sucrose was the most effective for the sequential dark (with Clostridium butyricum CGS5) and photo (with Rhodopseudomonas palutris WP3-5) fermentation process. The sequential dark-photo fermentation was stably operated for nearly 80 days, giving a maximum H2 yield of 11.61 mol H2/mol sucrose and a H2 production rate of 673.93 ml/h/l. The biogas produced from the sequential dark-photo fermentation (containing ca. 40.0% CO2) was directly fed into a microalga culture (Chlorella vulgaris C-C) cultivated at 30 °C under 60 μmol/m2/s illumination. The CO2 produced from the fermentation processes was completely consumed during the autotrophic growth of C. vulgaris C-C, resulting in a microalgal biomass concentration of 1999 mg/l composed mainly of 48.0% protein, 23.0% carbohydrate and 12.3% lipid.

Original languageEnglish
Pages (from-to)10944-10953
Number of pages10
JournalInternational Journal of Hydrogen Energy
Issue number20
Publication statusPublished - 2010 Oct

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


Dive into the research topics of 'Sequential dark-photo fermentation and autotrophic microalgal growth for high-yield and CO<sub>2</sub>-free biohydrogen production'. Together they form a unique fingerprint.

Cite this