Serotonin depresses excitatory synaptic transmission and depolarization-evoked Ca2+influx in rat basolateral amygdala via 5-HT1Areceptors

Li Ling Cheng, Su Jane Wang, Po Wu Gean

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Abstract

The actions of serotonin on rat basolateral amygdala neurons were studied with conventional intracellular recording techniques and fura-2 fluorimetric recordings. Bath application of 5-hydroxytryptamine (5-HT or serotonin) reversibly suppressed the excitatory postsynaptic potential in a concentration-dependent manner without affecting the resting membrane potential and neuronal input resistance. Extracellular Ba2+or pertussis toxin pretreatment did not affect the depressing effect of 5-HT suggesting that it is not mediated through activation of Gi/oprotein-coupled K+conductance. The sensitivity of postsynaptic neurons to glutamate receptor agonist was unaltered by the 5-HT pretreatment. In addition, the magnitude of paired-pulse facilitation was increased in the presence of 5-HT indicating a presynaptic mode of action. The effect of 5-HT was mimicked by the selective 5-HT1Aagonist 8-hydroxy-dipropylaminotetralin (8-OH-DPAT) and was blocked by the selective 5-HT1Aantagonist 1-(2-methoxyphenyl)-4[4-(2-phthalimido)butyl]piperazine oxadiazol-3-yl]methyl]phenyl]-methanesulphonamide. In contrast, the selective 5-HT2receptor antagonist ketanserin failed to affect the action of 5-HT. The effects of 5-HT and 8-OH-DPAT on the high K+-induced increase in [Ca2+]iwere studied in acutely dissociated basolateral amygdala neurons. High K+-induced increase in [Ca2+]iwas blocked by Ca2+-free solution and Cd2+suggesting that Ca2+entry responsible for the depolarizaton-evoked increase in [Ca2+]ioccurred through voltage-dependent Ca2+channels. Application of 5-HT and 8-OH-DPAT reduced the K+-induced Ca2+influx in a concentration-dependent manner. The effect of 5-HT was completely abolished in slices pretreated with Rp-cyclic adenosine 3;,5;-monophosphothioate (Rp-cAMP), a regulatory site antagonist of protein kinase A, suggesting that 5-HT may act through a cAMP-dependent mechanism. Taken together, these results suggest that functional 5-HT1Areceptors are present in the excitatory terminals and mediate the 5-HT inhibition of synaptic transmission in the amygdala.

Original languageEnglish
Pages (from-to)2163-2172
Number of pages10
JournalEuropean Journal of Neuroscience
Volume10
Issue number6
DOIs
Publication statusPublished - 1998

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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