Shear Stress Inhibits Homocysteine-Induced Stromal Cell-Derived Factor-1 Expression in Endothelial Cells

Mao Lin Sung, Chia Ching Wu, Hsin I. Chang, Chia Kuang Yen, Heng Jung Chen, Ju Chien Cheng, Shu Chien, Cheng Nan Chen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

RATIONALE:: Hyperhomocysteinemia contributes to vascular dysfunction and risks of cardiovascular diseases. Stromal cell-derived factor (SDF)-1, a chemokine expressed by endothelial cells (ECs), is highly expressed in advanced atherosclerotic lesions. The interplays among homocysteine, chemokines, and shear stress in regulating vascular endothelial function are not clearly understood. OBJECTIVE:: To investigate the mechanisms for modulations of EC SDF-1 expression by homocysteine and shear stress. METHODS AND RESULTS:: Homocysteine stimulation induced dose- and time-dependent SDF-1 expression and phosphorylation of mitogen-activated protein kinases extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. By using specific inhibitors, small interfering (si)RNA, and dominant negative mutants, we demonstrated that activation of JNK pathway is critical for the homocysteine-induced SDF-1 expression. Transcription factor ELISA and chromatin immunoprecipitation assays showed that homocysteine increased Sp1- and AP-1-DNA binding activities in ECs. Inhibition of Sp1 and AP-1 activations by specific siRNA blocked the homocysteine-induced SDF-1 promoter activity and expression. Preshearing of ECs for 1 to 4 hours at 20 dyn/cm inhibited the homocysteine-induced JNK phosphorylation, Sp1 and AP-1 activation, and SDF-1 expression. The homocysteine-induced SDF-1 expression was suppressed by NO donor. Inhibitor or siRNA for endothelial NO synthase abolished the shear inhibition of SDF-1 expression. CONCLUSIONS:: Our findings serve to elucidate the molecular mechanisms underlying the homocysteine induction of SDF-1 expression in ECs and the shear stress protection against this induction.

Original languageEnglish
Pages (from-to)755-763
Number of pages9
JournalCirculation Research
Volume105
Issue number8
DOIs
Publication statusPublished - 2009 Oct 1

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Chemokine CXCL12
Homocysteine
Endothelial Cells
JNK Mitogen-Activated Protein Kinases
Transcription Factor AP-1
Small Interfering RNA
Chemokines
Blood Vessels
Phosphorylation
Hyperhomocysteinemia
Critical Pathways
Chromatin Immunoprecipitation
Extracellular Signal-Regulated MAP Kinases
Mitogen-Activated Protein Kinases
Nitric Oxide Synthase
Transcription Factors
Cardiovascular Diseases
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Sung, Mao Lin ; Wu, Chia Ching ; Chang, Hsin I. ; Yen, Chia Kuang ; Chen, Heng Jung ; Cheng, Ju Chien ; Chien, Shu ; Chen, Cheng Nan. / Shear Stress Inhibits Homocysteine-Induced Stromal Cell-Derived Factor-1 Expression in Endothelial Cells. In: Circulation Research. 2009 ; Vol. 105, No. 8. pp. 755-763.
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Shear Stress Inhibits Homocysteine-Induced Stromal Cell-Derived Factor-1 Expression in Endothelial Cells. / Sung, Mao Lin; Wu, Chia Ching; Chang, Hsin I.; Yen, Chia Kuang; Chen, Heng Jung; Cheng, Ju Chien; Chien, Shu; Chen, Cheng Nan.

In: Circulation Research, Vol. 105, No. 8, 01.10.2009, p. 755-763.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Shear Stress Inhibits Homocysteine-Induced Stromal Cell-Derived Factor-1 Expression in Endothelial Cells

AU - Sung, Mao Lin

AU - Wu, Chia Ching

AU - Chang, Hsin I.

AU - Yen, Chia Kuang

AU - Chen, Heng Jung

AU - Cheng, Ju Chien

AU - Chien, Shu

AU - Chen, Cheng Nan

PY - 2009/10/1

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N2 - RATIONALE:: Hyperhomocysteinemia contributes to vascular dysfunction and risks of cardiovascular diseases. Stromal cell-derived factor (SDF)-1, a chemokine expressed by endothelial cells (ECs), is highly expressed in advanced atherosclerotic lesions. The interplays among homocysteine, chemokines, and shear stress in regulating vascular endothelial function are not clearly understood. OBJECTIVE:: To investigate the mechanisms for modulations of EC SDF-1 expression by homocysteine and shear stress. METHODS AND RESULTS:: Homocysteine stimulation induced dose- and time-dependent SDF-1 expression and phosphorylation of mitogen-activated protein kinases extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. By using specific inhibitors, small interfering (si)RNA, and dominant negative mutants, we demonstrated that activation of JNK pathway is critical for the homocysteine-induced SDF-1 expression. Transcription factor ELISA and chromatin immunoprecipitation assays showed that homocysteine increased Sp1- and AP-1-DNA binding activities in ECs. Inhibition of Sp1 and AP-1 activations by specific siRNA blocked the homocysteine-induced SDF-1 promoter activity and expression. Preshearing of ECs for 1 to 4 hours at 20 dyn/cm inhibited the homocysteine-induced JNK phosphorylation, Sp1 and AP-1 activation, and SDF-1 expression. The homocysteine-induced SDF-1 expression was suppressed by NO donor. Inhibitor or siRNA for endothelial NO synthase abolished the shear inhibition of SDF-1 expression. CONCLUSIONS:: Our findings serve to elucidate the molecular mechanisms underlying the homocysteine induction of SDF-1 expression in ECs and the shear stress protection against this induction.

AB - RATIONALE:: Hyperhomocysteinemia contributes to vascular dysfunction and risks of cardiovascular diseases. Stromal cell-derived factor (SDF)-1, a chemokine expressed by endothelial cells (ECs), is highly expressed in advanced atherosclerotic lesions. The interplays among homocysteine, chemokines, and shear stress in regulating vascular endothelial function are not clearly understood. OBJECTIVE:: To investigate the mechanisms for modulations of EC SDF-1 expression by homocysteine and shear stress. METHODS AND RESULTS:: Homocysteine stimulation induced dose- and time-dependent SDF-1 expression and phosphorylation of mitogen-activated protein kinases extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. By using specific inhibitors, small interfering (si)RNA, and dominant negative mutants, we demonstrated that activation of JNK pathway is critical for the homocysteine-induced SDF-1 expression. Transcription factor ELISA and chromatin immunoprecipitation assays showed that homocysteine increased Sp1- and AP-1-DNA binding activities in ECs. Inhibition of Sp1 and AP-1 activations by specific siRNA blocked the homocysteine-induced SDF-1 promoter activity and expression. Preshearing of ECs for 1 to 4 hours at 20 dyn/cm inhibited the homocysteine-induced JNK phosphorylation, Sp1 and AP-1 activation, and SDF-1 expression. The homocysteine-induced SDF-1 expression was suppressed by NO donor. Inhibitor or siRNA for endothelial NO synthase abolished the shear inhibition of SDF-1 expression. CONCLUSIONS:: Our findings serve to elucidate the molecular mechanisms underlying the homocysteine induction of SDF-1 expression in ECs and the shear stress protection against this induction.

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