Shorter daily dwelling time in peritoneal dialysis attenuates the epithelial-to-mesenchymal transition of mesothelial cells

Yi Che Lee, Yau Sheng Tsai, Shih Yuan Hung, Tsun Mei Lin, Sheng Hsiang Lin, Hung Hsiang Liou, Hsiang Chun Liu, Min Yu Chang, Hsi Hao Wang, Li Chun Ho, Yi Ting Chen, Hsin Pao Chen, Hong Arh Fan, Kuang Wen Liu, Yung Tang Kung, Hao Kuang Wang, Yuan Yow Chiou

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8 Citations (Scopus)


Background: Peritoneal dialysis (PD) therapy is known to induce morphological and functional changes in the peritoneal membrane. Long-term exposure to conventional bio-incompatible dialysate and peritonitis is the main etiology of inflammation. Consequently, the peritoneal membrane undergoes structural changes, including angiogenesis, fibrosis, and hyalinizing vasculopathy, which ultimately results in technique failure. The epithelial-to-mesenchymal transition (EMT) of mesothelial cells (MCs) plays an important role during the above process; however, the clinical parameters associated with the EMT process of MCs remain to be explored. Methods. To investigate the parameters impacting EMT during PD therapy, 53 clinical stable PD patients were enrolled. EMT assessments were conducted through human peritoneal MCs cultured from dialysate effluent with one consistent standard criterion (MC morphology and the expression of an epithelial marker, cytokeratin 18). The factors potentially associated with EMT were analyzed using logistic regression analysis. Primary MCs derived from the omentum were isolated for the in vitro study. Results: Forty-seven percent of the patients presented with EMT, 28% with non-EMT, and 15% with a mixed presentation. Logistic regression analysis showed that patients who received persistent PD therapy (dwelling time of 24 h/day) had significantly higher EMT tendency. These results were consistent in vitro. Conclusions: Dwelling time had a significant effect on the occurrence of EMT on MCs.

Original languageEnglish
Article number35
JournalBMC Nephrology
Issue number1
Publication statusPublished - 2014 Feb 20

All Science Journal Classification (ASJC) codes

  • Nephrology


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