Shrimp hemocytes release extracellular traps that kill bacteria

Tze Hann Ng, Sheng Hsiung Chang, Miao Hsien Wu, Han-Ching Wang

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Extracellular traps (ETs) are formed from the DNA, histones and cytoplasmic antimicrobial proteins that are released from a range of vertebrate immune-cells in response to pathogenic stimulation. This novel defense mechanism has not been demonstrated in invertebrates. In this study, we investigated the formation of ETs in the crustacean Litopenaeus vannamei. We found that stimulation of shrimp hemocytes with phorbol myristate acetate (PMA), lipopolysaccharide (LPS) and live Escherichia coli all led to the formation of the characteristic ET fibers made from host cell DNA. After E. coli stimulation, we found that histone proteins were co-localized with these extracellular DNA fibers. The results further showed that E. coli were trapped by these ET-like fibers and that some of the trapped bacteria were permeabilized. All of these results are characteristic of the ETs that are seen in vertebrates and we therefore conclude that shrimp are also capable of forming extracellular traps.

Original languageEnglish
Pages (from-to)644-651
Number of pages8
JournalDevelopmental and Comparative Immunology
Volume41
Issue number4
DOIs
Publication statusPublished - 2013 Dec 1

Fingerprint

Hemocytes
Bacteria
Escherichia coli
Histones
Vertebrates
DNA
Tetradecanoylphorbol Acetate
Invertebrates
Lipopolysaccharides
Extracellular Traps
Proteins

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Immunology

Cite this

Ng, Tze Hann ; Chang, Sheng Hsiung ; Wu, Miao Hsien ; Wang, Han-Ching. / Shrimp hemocytes release extracellular traps that kill bacteria. In: Developmental and Comparative Immunology. 2013 ; Vol. 41, No. 4. pp. 644-651.
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Shrimp hemocytes release extracellular traps that kill bacteria. / Ng, Tze Hann; Chang, Sheng Hsiung; Wu, Miao Hsien; Wang, Han-Ching.

In: Developmental and Comparative Immunology, Vol. 41, No. 4, 01.12.2013, p. 644-651.

Research output: Contribution to journalArticle

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