Shrimp laminin receptor binds with capsid proteins of two additional shrimp RNA viruses YHV and IMNV

Nattaphon Busayarat, Saengchan Senapin, Moltira Tonganunt, Kornsunee Phiwsaiya, Watcharachai Meemetta, Sasimanas Unajak, Sarawut Jitrapakdee, Chu Fang Lo, Amornrat Phongdara

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Laminin receptor (Lamr) in shrimp was previously proposed to be a potential receptor protein for Taura syndrome virus (TSV) based on yeast two-hybrid assays. Since shrimp Lamr bound to the VP1 capsid protein of TSV, we were interested to know whether capsid/envelope proteins from other shrimp viruses would also bind to Lamr. Thus, capsid/envelope encoding genes from 5 additional shrimp viruses were examined. These were Penaeus stylirostris densovirus (PstDNV), white spot syndrome virus (WSSV), infectious myonecrosis virus (IMNV), Macrobrachium rosenbergii nodavirus (MrNV), and yellow head virus (YHV). Protein interaction analysis using yeast two-hybrid assay revealed that Lamr specifically interacted with capsid/envelope proteins of RNA viruses IMNV and YHV but not MrNV and not with the capsid/envelope proteins of DNA viruses PstDNV and WSSV. In vitro pull-down assay also confirmed the interaction between Lamr and YHV gp116 envelope protein, and injection of recombinant Lamr (rLamr) protein produced in yeast cells protected shrimp against YHV in laboratory challenge tests.

Original languageEnglish
Pages (from-to)66-72
Number of pages7
JournalFish and Shellfish Immunology
Volume31
Issue number1
DOIs
Publication statusPublished - 2011 Jul

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

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