TY - JOUR
T1 - Shrimp laminin receptor binds with capsid proteins of two additional shrimp RNA viruses YHV and IMNV
AU - Busayarat, Nattaphon
AU - Senapin, Saengchan
AU - Tonganunt, Moltira
AU - Phiwsaiya, Kornsunee
AU - Meemetta, Watcharachai
AU - Unajak, Sasimanas
AU - Jitrapakdee, Sarawut
AU - Lo, Chu Fang
AU - Phongdara, Amornrat
N1 - Funding Information:
This work was supported by a Research-Team Strengthening Grant from the National Center for Genetic Engineering and Biotechnology , National Science and Technology Development Agency , Thailand and a National Research University grant. S.U. was the recipient of an RGJ-PhD scholarship from the Thailand Research Fund. The authors would like to thank Ms. Y. Thaowbut (Centex Shrimp) for skilled technical assistance, Dr. Y. Kawasaki (Osaka University, Japan) for providing pEG(KT) plasmid and yeast strain YK9, Prof. Dr. P. Sithigorngul (Srinakharinwirot University, Thailand) for giving us YHV antibodies, and Prof. T.W. Flegel (Centex Shrimp) for advice in preparing the manuscript.
PY - 2011/7
Y1 - 2011/7
N2 - Laminin receptor (Lamr) in shrimp was previously proposed to be a potential receptor protein for Taura syndrome virus (TSV) based on yeast two-hybrid assays. Since shrimp Lamr bound to the VP1 capsid protein of TSV, we were interested to know whether capsid/envelope proteins from other shrimp viruses would also bind to Lamr. Thus, capsid/envelope encoding genes from 5 additional shrimp viruses were examined. These were Penaeus stylirostris densovirus (PstDNV), white spot syndrome virus (WSSV), infectious myonecrosis virus (IMNV), Macrobrachium rosenbergii nodavirus (MrNV), and yellow head virus (YHV). Protein interaction analysis using yeast two-hybrid assay revealed that Lamr specifically interacted with capsid/envelope proteins of RNA viruses IMNV and YHV but not MrNV and not with the capsid/envelope proteins of DNA viruses PstDNV and WSSV. In vitro pull-down assay also confirmed the interaction between Lamr and YHV gp116 envelope protein, and injection of recombinant Lamr (rLamr) protein produced in yeast cells protected shrimp against YHV in laboratory challenge tests.
AB - Laminin receptor (Lamr) in shrimp was previously proposed to be a potential receptor protein for Taura syndrome virus (TSV) based on yeast two-hybrid assays. Since shrimp Lamr bound to the VP1 capsid protein of TSV, we were interested to know whether capsid/envelope proteins from other shrimp viruses would also bind to Lamr. Thus, capsid/envelope encoding genes from 5 additional shrimp viruses were examined. These were Penaeus stylirostris densovirus (PstDNV), white spot syndrome virus (WSSV), infectious myonecrosis virus (IMNV), Macrobrachium rosenbergii nodavirus (MrNV), and yellow head virus (YHV). Protein interaction analysis using yeast two-hybrid assay revealed that Lamr specifically interacted with capsid/envelope proteins of RNA viruses IMNV and YHV but not MrNV and not with the capsid/envelope proteins of DNA viruses PstDNV and WSSV. In vitro pull-down assay also confirmed the interaction between Lamr and YHV gp116 envelope protein, and injection of recombinant Lamr (rLamr) protein produced in yeast cells protected shrimp against YHV in laboratory challenge tests.
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U2 - 10.1016/j.fsi.2011.03.007
DO - 10.1016/j.fsi.2011.03.007
M3 - Article
C2 - 21414409
AN - SCOPUS:79957875645
SN - 1050-4648
VL - 31
SP - 66
EP - 72
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
IS - 1
ER -