TY - JOUR
T1 - Shrimp Pm-fortilin inhibits the expression of early and late genes of white spot syndrome virus (WSSV) in an insect cell model
AU - Nupan, Benjamas
AU - Phongdara, Amornrat
AU - Saengsakda, Manasawan
AU - Leu, Jiann Horng
AU - Lo, Chu Fang
N1 - Funding Information:
This research was supported by a grant from The Office of the Higher Education Commission, Thailand . Ms. Benjamas Nupan was supported by CHE Ph.D. Scholarship and Ms. Manasawan Saengsakda was supported by CHE Postdoctoral Scholarship. We are grateful to Prof. Dr. T.W. Flegel, Centex Shrimp, Mahidol University, Thailand, for critical reading and editing the manuscript. We also thank Dr. Brian Hodgson for assistance with English language.
PY - 2011/4
Y1 - 2011/4
N2 - Fortilin plays an important role in anti-apoptotic mechanisms and cell proliferation in many eukaryotic organisms. This work confirmed previous reports that Sf9 can support the replication of white spot syndrome virus (WSSV) genomic material by using immunohistochemistry with a specific antibody to detect the immediate early gene 1 (ie1) and by amplification of WSSV DNA and mRNA products. Using this insect-cell model system, we show that overexpression of Pm-fortilin in Sf9 cells inhibited the expression of WSSV early genes and late genes (WSSV- DNA polymerase, VP15 and VP28) but not an immediate early gene ie1. This is the first time that an insect cell line has been used to demonstrate interaction between a shrimp gene and genes of a shrimp virus.
AB - Fortilin plays an important role in anti-apoptotic mechanisms and cell proliferation in many eukaryotic organisms. This work confirmed previous reports that Sf9 can support the replication of white spot syndrome virus (WSSV) genomic material by using immunohistochemistry with a specific antibody to detect the immediate early gene 1 (ie1) and by amplification of WSSV DNA and mRNA products. Using this insect-cell model system, we show that overexpression of Pm-fortilin in Sf9 cells inhibited the expression of WSSV early genes and late genes (WSSV- DNA polymerase, VP15 and VP28) but not an immediate early gene ie1. This is the first time that an insect cell line has been used to demonstrate interaction between a shrimp gene and genes of a shrimp virus.
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U2 - 10.1016/j.dci.2010.11.016
DO - 10.1016/j.dci.2010.11.016
M3 - Article
C2 - 21130805
AN - SCOPUS:79951949642
SN - 0145-305X
VL - 35
SP - 469
EP - 475
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 4
ER -