Significance of migration-related genes (S100A9, MAGED4, C8orf30A, IL-8) in esophageal squamous cell carcinoma

Ching Tang Huang, Guan Cheng Huang, Pey Shan Wu, Li Wha Wu, Siao Han Lin, Wu Wei Lai, Yi Ching Wang, Hsiao Sheng Liu

Research output: Contribution to journalArticle

Abstract

To identify any biomarkers related to the migration of esophageal squamous cell carcinoma (ESCC) cells, ESCC CE81T cells were used to establish the CE81T-1 subline, which demonstrates increased migration activity after Transwell screening and microarray analysis. Among the differentially expressed genes, S100A9 was most downregulated, and MAGED4, C8orf30A, and IL-8 were the most upregulated in CE81T-1 cells. The expression of these four genes at the mRNA level was validated using the ESCC CE81T and KYSE cell lines and clarified in ESCC specimens using real-time polymerase chain reaction. Among 60 pairs of ESCC specimens (normal and tumor specimens), the expression level of S100A9 mRNA was significantly lower in the tumor sections in comparison with the normal sections (p= 0.0228). In contrast, the expression level of IL-8 mRNA was significantly higher in the tumor sections in comparison with the normal sections (p= 0.0061). Furthermore, C8orf30A expression was significantly correlated with ESCC metastatic status (p= 0.0358) and associated with poorer survival (p= 0.036), as determined by Kaplan-Meier analysis. Functional studies revealed that S100A9 plays a suppressive role in the proliferation and migration of ESCC cells through the overexpression of ectopic S100A9 and small interfering RNA, as determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assays. Altogether, this study reveals that C8orf30A has the potential to be used as a novel biomarker for the prognosis for ESCC metastasis and survival. Furthermore, the IL-8 and S100A9 genes may have potential in ESCC diagnosis.

Original languageEnglish
Pages (from-to)16-18
Number of pages3
JournalGenomic Medicine, Biomarkers, and Health Sciences
Volume4
Issue number1-2
DOIs
Publication statusPublished - 2012 Mar 1

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Interleukin-8
Genes
Tumors
Biomarkers
Messenger RNA
Cells
Epithelial Cells
Esophageal Squamous Cell Carcinoma
Neoplasms
Polymerase chain reaction
Kaplan-Meier Estimate
Microarray Analysis
Microarrays
RNA
Small Interfering RNA
Real-Time Polymerase Chain Reaction
Assays
Screening
Down-Regulation
Neoplasm Metastasis

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology(all)
  • Drug Discovery

Cite this

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title = "Significance of migration-related genes (S100A9, MAGED4, C8orf30A, IL-8) in esophageal squamous cell carcinoma",
abstract = "To identify any biomarkers related to the migration of esophageal squamous cell carcinoma (ESCC) cells, ESCC CE81T cells were used to establish the CE81T-1 subline, which demonstrates increased migration activity after Transwell screening and microarray analysis. Among the differentially expressed genes, S100A9 was most downregulated, and MAGED4, C8orf30A, and IL-8 were the most upregulated in CE81T-1 cells. The expression of these four genes at the mRNA level was validated using the ESCC CE81T and KYSE cell lines and clarified in ESCC specimens using real-time polymerase chain reaction. Among 60 pairs of ESCC specimens (normal and tumor specimens), the expression level of S100A9 mRNA was significantly lower in the tumor sections in comparison with the normal sections (p= 0.0228). In contrast, the expression level of IL-8 mRNA was significantly higher in the tumor sections in comparison with the normal sections (p= 0.0061). Furthermore, C8orf30A expression was significantly correlated with ESCC metastatic status (p= 0.0358) and associated with poorer survival (p= 0.036), as determined by Kaplan-Meier analysis. Functional studies revealed that S100A9 plays a suppressive role in the proliferation and migration of ESCC cells through the overexpression of ectopic S100A9 and small interfering RNA, as determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assays. Altogether, this study reveals that C8orf30A has the potential to be used as a novel biomarker for the prognosis for ESCC metastasis and survival. Furthermore, the IL-8 and S100A9 genes may have potential in ESCC diagnosis.",
author = "Huang, {Ching Tang} and Huang, {Guan Cheng} and Wu, {Pey Shan} and Wu, {Li Wha} and Lin, {Siao Han} and Lai, {Wu Wei} and Wang, {Yi Ching} and Liu, {Hsiao Sheng}",
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Significance of migration-related genes (S100A9, MAGED4, C8orf30A, IL-8) in esophageal squamous cell carcinoma. / Huang, Ching Tang; Huang, Guan Cheng; Wu, Pey Shan; Wu, Li Wha; Lin, Siao Han; Lai, Wu Wei; Wang, Yi Ching; Liu, Hsiao Sheng.

In: Genomic Medicine, Biomarkers, and Health Sciences, Vol. 4, No. 1-2, 01.03.2012, p. 16-18.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Significance of migration-related genes (S100A9, MAGED4, C8orf30A, IL-8) in esophageal squamous cell carcinoma

AU - Huang, Ching Tang

AU - Huang, Guan Cheng

AU - Wu, Pey Shan

AU - Wu, Li Wha

AU - Lin, Siao Han

AU - Lai, Wu Wei

AU - Wang, Yi Ching

AU - Liu, Hsiao Sheng

PY - 2012/3/1

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N2 - To identify any biomarkers related to the migration of esophageal squamous cell carcinoma (ESCC) cells, ESCC CE81T cells were used to establish the CE81T-1 subline, which demonstrates increased migration activity after Transwell screening and microarray analysis. Among the differentially expressed genes, S100A9 was most downregulated, and MAGED4, C8orf30A, and IL-8 were the most upregulated in CE81T-1 cells. The expression of these four genes at the mRNA level was validated using the ESCC CE81T and KYSE cell lines and clarified in ESCC specimens using real-time polymerase chain reaction. Among 60 pairs of ESCC specimens (normal and tumor specimens), the expression level of S100A9 mRNA was significantly lower in the tumor sections in comparison with the normal sections (p= 0.0228). In contrast, the expression level of IL-8 mRNA was significantly higher in the tumor sections in comparison with the normal sections (p= 0.0061). Furthermore, C8orf30A expression was significantly correlated with ESCC metastatic status (p= 0.0358) and associated with poorer survival (p= 0.036), as determined by Kaplan-Meier analysis. Functional studies revealed that S100A9 plays a suppressive role in the proliferation and migration of ESCC cells through the overexpression of ectopic S100A9 and small interfering RNA, as determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assays. Altogether, this study reveals that C8orf30A has the potential to be used as a novel biomarker for the prognosis for ESCC metastasis and survival. Furthermore, the IL-8 and S100A9 genes may have potential in ESCC diagnosis.

AB - To identify any biomarkers related to the migration of esophageal squamous cell carcinoma (ESCC) cells, ESCC CE81T cells were used to establish the CE81T-1 subline, which demonstrates increased migration activity after Transwell screening and microarray analysis. Among the differentially expressed genes, S100A9 was most downregulated, and MAGED4, C8orf30A, and IL-8 were the most upregulated in CE81T-1 cells. The expression of these four genes at the mRNA level was validated using the ESCC CE81T and KYSE cell lines and clarified in ESCC specimens using real-time polymerase chain reaction. Among 60 pairs of ESCC specimens (normal and tumor specimens), the expression level of S100A9 mRNA was significantly lower in the tumor sections in comparison with the normal sections (p= 0.0228). In contrast, the expression level of IL-8 mRNA was significantly higher in the tumor sections in comparison with the normal sections (p= 0.0061). Furthermore, C8orf30A expression was significantly correlated with ESCC metastatic status (p= 0.0358) and associated with poorer survival (p= 0.036), as determined by Kaplan-Meier analysis. Functional studies revealed that S100A9 plays a suppressive role in the proliferation and migration of ESCC cells through the overexpression of ectopic S100A9 and small interfering RNA, as determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assays. Altogether, this study reveals that C8orf30A has the potential to be used as a novel biomarker for the prognosis for ESCC metastasis and survival. Furthermore, the IL-8 and S100A9 genes may have potential in ESCC diagnosis.

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