Simple, effective protein extraction method and proteomics analysis from polyunsaturated fatty acids-producing micro-organisms

Xueping Ling, Jing Guo, Chuqiang Zheng, Chiming Ye, Yinghua Lu, Xueshan Pan, Zhengqi Chen, I. Son Ng

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Polyunsaturated fatty acids (PUFAs) are valuable ingredients in the food and pharmaceutical products due to their beneficial influence on human health. Most studies paid attention on the production of PUFAs from oleaginous micro-organisms but seldom on the comparative proteomics of cells. In the study, three methods (i.e., cold shock, acetone precipitation and ethanol precipitation) for lipid removal from crude protein extracts were applied in different PUFAs-producing micro-organisms. Among the selective strains, Schizochytrium was used as an oleaginous strain with high lipid of 60.3 (w/w %) in bio-mass. The Mortierella alpina and Cunninghamella echin-ulata were chosen as the low-lipid-content strains with 25.8 (w/w %) and 21.8 (w/w %) of lipid in biomass, respec-tively. The cold shock resulted as the most effective method for lipid removed, thus obtained higher protein amount for Schizochytrium. Moreover, from the comparative proteomics for the three PUFAs-producing strains, it showed more significant proteins of up or down-regulation were explored under cold shock treatment. Therefore, the essential proteins (i.e., polyunsaturated fatty acid synthase) and regulating proteins were observed. In conclusion, this study provides a valuable and practical approach for analysis of high PUFAs-producing strains at the proteomics level, and would further accelerate the understanding of the metabolic flux in oleaginous micro-organisms.

Original languageEnglish
Pages (from-to)2331-2341
Number of pages11
JournalBioprocess and biosystems engineering
Volume38
Issue number12
DOIs
Publication statusPublished - 2015 Dec

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering

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