Simulation and experimental validation of micro polymerase chain reaction chips

Yu-Cheng Lin, Chung Che Yang, Ming Yuan Huang

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

This study used finite element analysis to simulate the temperature characteristics of a micro polymerase chain reaction (PCR) chip. The micro-PCR chip was fabricated on a silicon wafer and Pyrex glass using photolithography, wet etching, and anodic bonding methods. The main goal of this study was to analyze the temperature uniformity and distribution of the micro-PCR chip, the temperature distribution of the DNA sample, and the transient temperature difference between the heater and DNA sample. The finite element analysis results were also confirmed by one-dimensional theoretic analysis. The simulation results were used to improve the thermal cycling time of a rapid micro-PCR system, consisting of a rapid thermal cycling system and a micro-PCR chip. The improved thermal cycles of the rapid μPCR system were verified using serum samples from patients with chronic hepatitis C. The hepatitis C virus (HCV) amplicon of the rapid μPCR system was analyzed by slab gel electrophoresis with DNA marker separation in parallel.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume71
Issue number1-2
DOIs
Publication statusPublished - 2000 Nov 15

Fingerprint

polymerase chain reaction
Polymerase chain reaction
chips
hepatitis
simulation
DNA
deoxyribonucleic acid
Thermal cycling
cycles
Finite element method
Wet etching
dimensional analysis
borosilicate glass
viruses
Photolithography
electrophoresis
photolithography
Electrophoresis
Silicon wafers
Viruses

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Lin, Yu-Cheng ; Yang, Chung Che ; Huang, Ming Yuan. / Simulation and experimental validation of micro polymerase chain reaction chips. In: Sensors and Actuators, B: Chemical. 2000 ; Vol. 71, No. 1-2. pp. 127-133.
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Simulation and experimental validation of micro polymerase chain reaction chips. / Lin, Yu-Cheng; Yang, Chung Che; Huang, Ming Yuan.

In: Sensors and Actuators, B: Chemical, Vol. 71, No. 1-2, 15.11.2000, p. 127-133.

Research output: Contribution to journalArticle

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