TY - GEN
T1 - Simulation and experimental verification of micro polymerase chain reaction chips
AU - Lin, Yu-Cheng
AU - Yang, Chung Che
AU - Hwang, Ming Yuan
AU - Chang, Yao Te
PY - 2000/12/1
Y1 - 2000/12/1
N2 - This study used finite element analysis to simulate the temperature characteristics of a micro polymerase chain reaction (PCR) chip. The micro-PCR chip was fabricated on a silicon wafer and Pyrex glass using photolithography, wet etching, and anodic bonding methods. The main goal of this study was to analyze the temperature uniformity and distribution of the micro-PCR chip, the temperature distribution of the DNA sample, and the transient temperature difference between the heater and DNA sample. The finite element analysis results were also confirmed by one-dimensional theoretic analysis. The simulation results were used to improve the thermal cycling time of a rapid micro-PCR system, consisting of a rapid thermal cycling system and a micro-PCR chip. The improved thermal cycles of the rapid μPCR system were verified using serum samples from patients with chronic hepatitis C. The hepatitis C virus (HCV) amplicon of the rapid μPCR system was analyzed by slab gel electrophoresis with DNA marker separation in parallel.
AB - This study used finite element analysis to simulate the temperature characteristics of a micro polymerase chain reaction (PCR) chip. The micro-PCR chip was fabricated on a silicon wafer and Pyrex glass using photolithography, wet etching, and anodic bonding methods. The main goal of this study was to analyze the temperature uniformity and distribution of the micro-PCR chip, the temperature distribution of the DNA sample, and the transient temperature difference between the heater and DNA sample. The finite element analysis results were also confirmed by one-dimensional theoretic analysis. The simulation results were used to improve the thermal cycling time of a rapid micro-PCR system, consisting of a rapid thermal cycling system and a micro-PCR chip. The improved thermal cycles of the rapid μPCR system were verified using serum samples from patients with chronic hepatitis C. The hepatitis C virus (HCV) amplicon of the rapid μPCR system was analyzed by slab gel electrophoresis with DNA marker separation in parallel.
UR - http://www.scopus.com/inward/record.url?scp=6344290158&partnerID=8YFLogxK
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M3 - Conference contribution
AN - SCOPUS:6344290158
SN - 0966613570
T3 - 2000 International Conference on Modeling and Simulation of Microsystems - MSM 2000
SP - 648
EP - 651
BT - 2000 International Conference on Modeling and Simulation of Microsystems - MSM 2000
A2 - Laudon, M.
A2 - Romanowicz, B.
T2 - 2000 International Conference on Modeling and Simulation of Microsystems - MSM 2000
Y2 - 27 March 2000 through 29 March 2000
ER -
