TY - JOUR
T1 - Simultaneous 17β-estradiol degradation, carbon dioxide fixation, and carotenoid accumulation by Thermosynechococcus sp. CL-1
AU - Narindri Rara Winayu, Birgitta
AU - Chang, Yu Ling
AU - Hsueh, Hsin Ta
AU - Chu, Hsin
N1 - Funding Information:
Respectful acknowledgement from the authors for the Taiwan Ministry of Science and Technology’s financial support [Project number: NSC 102-2622-E-006-006-MY3]
Publisher Copyright:
© 2022
PY - 2022/6
Y1 - 2022/6
N2 - Thermosynechococcus sp. CL-1 (TCL-1) has a high potency to utilize CO2 under extreme conditions including high temperature, alkaline condition, and the occurrence of 17β-estradiol (E2). In this study, TCL-1 cultivation with E2 addition in the range of 0–20 mg/L was combined with various growth arrangements (light intensity and dissolved inorganic nitrogen/DIN level). After 120 h cultivation, the 1.0 mg/L E2, 200 µmol photons/m2/s light intensity, and 5.8 mM available nitrogen performed the best growth with 4.58 ± 0.18 mg/L/h biomass productivity, 94.9 ± 3.3% total estrogen removal, and 11.41 ± 0.11 mg/L/h CO2 fixation rate. Estrogen degradation was mainly carried out by biodegradation route which started from E2 conversion into estrone/E1 and with only 4–6% influence from the abiotic factors. Compared with the accumulated zeaxanthin, β-carotene was dominantly generated with a productivity of 0.043 ± 0.019 mg/L/h. Therefore, TCL-1 cultivation is an efficient strategy for simultaneous CO2 fixation, estrogen removal, and carotenoid accumulation as valuable byproducts.
AB - Thermosynechococcus sp. CL-1 (TCL-1) has a high potency to utilize CO2 under extreme conditions including high temperature, alkaline condition, and the occurrence of 17β-estradiol (E2). In this study, TCL-1 cultivation with E2 addition in the range of 0–20 mg/L was combined with various growth arrangements (light intensity and dissolved inorganic nitrogen/DIN level). After 120 h cultivation, the 1.0 mg/L E2, 200 µmol photons/m2/s light intensity, and 5.8 mM available nitrogen performed the best growth with 4.58 ± 0.18 mg/L/h biomass productivity, 94.9 ± 3.3% total estrogen removal, and 11.41 ± 0.11 mg/L/h CO2 fixation rate. Estrogen degradation was mainly carried out by biodegradation route which started from E2 conversion into estrone/E1 and with only 4–6% influence from the abiotic factors. Compared with the accumulated zeaxanthin, β-carotene was dominantly generated with a productivity of 0.043 ± 0.019 mg/L/h. Therefore, TCL-1 cultivation is an efficient strategy for simultaneous CO2 fixation, estrogen removal, and carotenoid accumulation as valuable byproducts.
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U2 - 10.1016/j.biortech.2022.127197
DO - 10.1016/j.biortech.2022.127197
M3 - Article
C2 - 35460842
AN - SCOPUS:85129516765
SN - 0960-8524
VL - 354
JO - Bioresource technology
JF - Bioresource technology
M1 - 127197
ER -