Simultaneous detection of multiple NT-proBNP clinical samples utilizing an aptamer-based sandwich assay on an integrated microfluidic system

Anirban Sinha, Priya Gopinathan, Yi Da Chung, Shu-Chu Shiesh, Gwo Bin Lee

Research output: Contribution to journalArticle

Abstract

Although cardiovascular diseases such as heart failure (HF) affect 30 million people globally, the early detection of HF has, until recently, been difficult and prone to misdiagnoses. Monitoring the circulatory levels of a relatively new biomarker, the N-terminal prohormone of a B-type natriuretic peptide, could be used for early risk evaluation of HF. Therefore, we developed a pneumatically-driven, automatic integrated microfluidic platform equipped with micromixers, micropumps, and microvalves for the simultaneous detection of NT-proBNP in up to six clinical samples within 25 min by using a novel aptamer-based sandwich assay, and the limit of detection was only 1.53 pg mL-1; given that the chip is 64% more compact than those developed in our prior works and requires only 5 μL of sample input, it may serve as a promising tool for early diagnosis of HF.

Original languageEnglish
Pages (from-to)1676-1685
Number of pages10
JournalLab on a Chip
Volume19
Issue number9
DOIs
Publication statusPublished - 2019 Jan 1

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Microfluidics
Assays
Heart Failure
Brain Natriuretic Peptide
Biomarkers
Diagnostic Errors
Limit of Detection
Early Diagnosis
Cardiovascular Diseases
pro-brain natriuretic peptide (1-76)
Monitoring

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

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abstract = "Although cardiovascular diseases such as heart failure (HF) affect 30 million people globally, the early detection of HF has, until recently, been difficult and prone to misdiagnoses. Monitoring the circulatory levels of a relatively new biomarker, the N-terminal prohormone of a B-type natriuretic peptide, could be used for early risk evaluation of HF. Therefore, we developed a pneumatically-driven, automatic integrated microfluidic platform equipped with micromixers, micropumps, and microvalves for the simultaneous detection of NT-proBNP in up to six clinical samples within 25 min by using a novel aptamer-based sandwich assay, and the limit of detection was only 1.53 pg mL-1; given that the chip is 64{\%} more compact than those developed in our prior works and requires only 5 μL of sample input, it may serve as a promising tool for early diagnosis of HF.",
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Simultaneous detection of multiple NT-proBNP clinical samples utilizing an aptamer-based sandwich assay on an integrated microfluidic system. / Sinha, Anirban; Gopinathan, Priya; Chung, Yi Da; Shiesh, Shu-Chu; Lee, Gwo Bin.

In: Lab on a Chip, Vol. 19, No. 9, 01.01.2019, p. 1676-1685.

Research output: Contribution to journalArticle

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