Simultaneous determination of p-phenylenediamines and aminophenols in hair dye products

Interlaboratory study

Yu Chun Yeh, Hui Jung Wu, Wei Sheng Huang, Cheng Chin Lin, Yu Pen Chen, Mei Chyun Tzou, Jui-Chen Tsai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An isocratic HPLC method routinely used in the National Laboratory for Food and Drug Analysis of Taiwan was validated for the simultaneous determination of six aminophenols and phenylenediamines in commercial hair dyes. After extraction of the commercial hair dye product, the dye intermediates were determined by HPLC. Recoveries from the extraction were between 91.6 and 96.5%. The method was then evaluated in an interlaboratory collaborative study according to AOAC guidelines. Five laboratories in Taiwan participated in the study that analyzed the test product, which was preanalyzed by two laboratories to ensure acceptable homogeneity. The RSDr and RSDR values of the measurements obtained for the dye intermediates in the product were ≤3.75 and ≤5.95%, respectively. The method demonstrated acceptable reproducibility, as evidenced by HorRat values of 0.82-0.97. The applicability of the method to the determination of oxidative hair dye components was further demonstrated in analyses of two different products. The method is thus proposed to be used by manufacturers and laboratories to evaluate the quality of commercial hair dyes containing the six aminophenols and phenylenediamines.

Original languageEnglish
Pages (from-to)650-654
Number of pages5
JournalJournal of AOAC International
Volume94
Issue number2
Publication statusPublished - 2011 Mar 1

Fingerprint

aminophenols
Hair Dyes
hair
hairs
dyes
dye
Aminophenols
Phenylenediamines
Coloring Agents
Taiwan
High Pressure Liquid Chromatography
Food Analysis
methodology
Recovery
reproducibility
homogeneity
4-phenylenediamine
4-aminophenol
product
Guidelines

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Environmental Chemistry
  • Agronomy and Crop Science
  • Pharmacology

Cite this

Yeh, Y. C., Wu, H. J., Huang, W. S., Lin, C. C., Chen, Y. P., Tzou, M. C., & Tsai, J-C. (2011). Simultaneous determination of p-phenylenediamines and aminophenols in hair dye products: Interlaboratory study. Journal of AOAC International, 94(2), 650-654.
Yeh, Yu Chun ; Wu, Hui Jung ; Huang, Wei Sheng ; Lin, Cheng Chin ; Chen, Yu Pen ; Tzou, Mei Chyun ; Tsai, Jui-Chen. / Simultaneous determination of p-phenylenediamines and aminophenols in hair dye products : Interlaboratory study. In: Journal of AOAC International. 2011 ; Vol. 94, No. 2. pp. 650-654.
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Simultaneous determination of p-phenylenediamines and aminophenols in hair dye products : Interlaboratory study. / Yeh, Yu Chun; Wu, Hui Jung; Huang, Wei Sheng; Lin, Cheng Chin; Chen, Yu Pen; Tzou, Mei Chyun; Tsai, Jui-Chen.

In: Journal of AOAC International, Vol. 94, No. 2, 01.03.2011, p. 650-654.

Research output: Contribution to journalArticle

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