Simultaneous nutrient removal and lipid production with Chlorella vulgaris on sterilized and non-sterilized anaerobically pretreated piggery wastewater

Jatta M. Marjakangas, Chun-Yen Chen, Aino Maija Lakaniemi, Jaakko A. Puhakka, Liang-Ming Whang, Jo-Shu Chang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Piggery wastewater is a potent nutrient source for microalgal lipid production. Wastewater has been usually sterilized when used for microalgal cultivation. This is uneconomical in large-scale applications. Therefore, lipid productivity of Chlorella vulgaris CY5 using sterilized and non-sterilized diluted anaerobically pretreated piggery wastewater was studied in batch reactors. The maximum average lipid productivity was obtained after 12 days of incubation and it was higher with the sterilized wastewater than with the non-sterilized one (117g/L/d vs. 91.3g/L/d), due to the higher biomass concentration. Because of the unexpected increase of dissolved organic carbon (DOC) in the cultures, second experiment was conducted to characterize the composition of produced DOC in non-sterilized wastewater. Carbohydrate content increased in the liquid phase but decreased in the biomass after nitrogen had been exhausted. After 12 days of incubation, soluble chemical oxygen demand (CODs) was 414±56mg/L, biomass production was 2.8±0.15g/L, and lipid content was 30.3±1.2wt%. Average lipid productivity from day zero to day 12 was 70.5±1.1g/L/d. C. vulgaris removed nutrients from the non-sterilized wastewater and produced oleaginous biomass, although the lipid productivity was higher with sterilized wastewater.

Original languageEnglish
Pages (from-to)177-184
Number of pages8
JournalBiochemical Engineering Journal
Volume103
DOIs
Publication statusPublished - 2015 Nov 5

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Chlorella vulgaris
Waste Water
Lipids
Nutrients
Wastewater
Food
Biomass
Productivity
Organic carbon
Carbon
Biological Oxygen Demand Analysis
Chemical oxygen demand
Batch reactors
Carbohydrates
Nitrogen
Liquids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

Cite this

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title = "Simultaneous nutrient removal and lipid production with Chlorella vulgaris on sterilized and non-sterilized anaerobically pretreated piggery wastewater",
abstract = "Piggery wastewater is a potent nutrient source for microalgal lipid production. Wastewater has been usually sterilized when used for microalgal cultivation. This is uneconomical in large-scale applications. Therefore, lipid productivity of Chlorella vulgaris CY5 using sterilized and non-sterilized diluted anaerobically pretreated piggery wastewater was studied in batch reactors. The maximum average lipid productivity was obtained after 12 days of incubation and it was higher with the sterilized wastewater than with the non-sterilized one (117g/L/d vs. 91.3g/L/d), due to the higher biomass concentration. Because of the unexpected increase of dissolved organic carbon (DOC) in the cultures, second experiment was conducted to characterize the composition of produced DOC in non-sterilized wastewater. Carbohydrate content increased in the liquid phase but decreased in the biomass after nitrogen had been exhausted. After 12 days of incubation, soluble chemical oxygen demand (CODs) was 414±56mg/L, biomass production was 2.8±0.15g/L, and lipid content was 30.3±1.2wt{\%}. Average lipid productivity from day zero to day 12 was 70.5±1.1g/L/d. C. vulgaris removed nutrients from the non-sterilized wastewater and produced oleaginous biomass, although the lipid productivity was higher with sterilized wastewater.",
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Simultaneous nutrient removal and lipid production with Chlorella vulgaris on sterilized and non-sterilized anaerobically pretreated piggery wastewater. / Marjakangas, Jatta M.; Chen, Chun-Yen; Lakaniemi, Aino Maija; Puhakka, Jaakko A.; Whang, Liang-Ming; Chang, Jo-Shu.

In: Biochemical Engineering Journal, Vol. 103, 05.11.2015, p. 177-184.

Research output: Contribution to journalArticle

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AU - Marjakangas, Jatta M.

AU - Chen, Chun-Yen

AU - Lakaniemi, Aino Maija

AU - Puhakka, Jaakko A.

AU - Whang, Liang-Ming

AU - Chang, Jo-Shu

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