Simultaneous real-time quantification of blood flow and vascular growth in the chick embryo using optical coherence tomography

William J. Kowalski, Nikola C. Teslovich, Chia-Yuan Chen, Bradley B. Keller, Kerem Pekkan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

Experimental and clinical data indicate that hemodynamic forces within the embryo provide critical biomechanical cues for cardiovascular morphogenesis, growth, and remodeling and that perturbed flow is a major etiology of congenital heart disease. However, embryonic flow-growth relationships are largely qualitative and poorly defined. In this work, we provide a quantitative analysis of in vivo flow and growth trends in the chick embryo using optical coherence tomography (OCT) to acquire simultaneous velocity and structural data of the right vitelline artery continuously over a ten hour period beginning at stage 16 (hour 54). We obtained 3D vessel volumes (15 μm lateral, 4.3 μm axial resolutions, 6 μm slice spacing) at 60 minute intervals, taking a B-scan time series totaling one cardiac cycle at each slice. Embryos were maintained at a constant 37°C and 60% humidity during the entire acquisition period through an inhouse built chamber. The 3D vessel lumen geometries were reconstructed manually to assess growth. Blood flow velocity was computed from the central B-scan using red blood cell particle image velocimetry. The use of extended OCT imaging as a non-invasive method for continuous and simultaneous flow and structural data can enhance our understanding of the biomechanical regulation of critical events in morphogenesis. Data acquired will be useful to validate predictive finite-element 3D growth models..

Original languageEnglish
Title of host publicationOptical Methods in Developmental Biology II
PublisherSPIE
Volume8953
ISBN (Print)9780819498663
DOIs
Publication statusPublished - 2014
EventOptical Methods in Developmental Biology II - San Francisco, CA, United States
Duration: 2014 Feb 12014 Feb 2

Other

OtherOptical Methods in Developmental Biology II
CountryUnited States
CitySan Francisco, CA
Period14-02-0114-02-02

Fingerprint

embryos
Optical tomography
Optical Coherence Tomography
Chick Embryo
blood flow
Blood Vessels
Blood
tomography
Growth
Morphogenesis
vessels
Embryonic Structures
etiology
heart diseases
hemodynamics
lumens
Blood Flow Velocity
Rheology
cues
Hemodynamics

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Kowalski, W. J., Teslovich, N. C., Chen, C-Y., Keller, B. B., & Pekkan, K. (2014). Simultaneous real-time quantification of blood flow and vascular growth in the chick embryo using optical coherence tomography. In Optical Methods in Developmental Biology II (Vol. 8953). [895307] SPIE. https://doi.org/10.1117/12.2044932
Kowalski, William J. ; Teslovich, Nikola C. ; Chen, Chia-Yuan ; Keller, Bradley B. ; Pekkan, Kerem. / Simultaneous real-time quantification of blood flow and vascular growth in the chick embryo using optical coherence tomography. Optical Methods in Developmental Biology II. Vol. 8953 SPIE, 2014.
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Kowalski, WJ, Teslovich, NC, Chen, C-Y, Keller, BB & Pekkan, K 2014, Simultaneous real-time quantification of blood flow and vascular growth in the chick embryo using optical coherence tomography. in Optical Methods in Developmental Biology II. vol. 8953, 895307, SPIE, Optical Methods in Developmental Biology II, San Francisco, CA, United States, 14-02-01. https://doi.org/10.1117/12.2044932

Simultaneous real-time quantification of blood flow and vascular growth in the chick embryo using optical coherence tomography. / Kowalski, William J.; Teslovich, Nikola C.; Chen, Chia-Yuan; Keller, Bradley B.; Pekkan, Kerem.

Optical Methods in Developmental Biology II. Vol. 8953 SPIE, 2014. 895307.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Kowalski WJ, Teslovich NC, Chen C-Y, Keller BB, Pekkan K. Simultaneous real-time quantification of blood flow and vascular growth in the chick embryo using optical coherence tomography. In Optical Methods in Developmental Biology II. Vol. 8953. SPIE. 2014. 895307 https://doi.org/10.1117/12.2044932