Simultaneous real-time quantification of blood flow and vascular growth in the chick embryo using optical coherence tomography

William J. Kowalski, Nikola C. Teslovich, Chia Yuan Chen, Bradley B. Keller, Kerem Pekkan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

Experimental and clinical data indicate that hemodynamic forces within the embryo provide critical biomechanical cues for cardiovascular morphogenesis, growth, and remodeling and that perturbed flow is a major etiology of congenital heart disease. However, embryonic flow-growth relationships are largely qualitative and poorly defined. In this work, we provide a quantitative analysis of in vivo flow and growth trends in the chick embryo using optical coherence tomography (OCT) to acquire simultaneous velocity and structural data of the right vitelline artery continuously over a ten hour period beginning at stage 16 (hour 54). We obtained 3D vessel volumes (15 μm lateral, 4.3 μm axial resolutions, 6 μm slice spacing) at 60 minute intervals, taking a B-scan time series totaling one cardiac cycle at each slice. Embryos were maintained at a constant 37°C and 60% humidity during the entire acquisition period through an inhouse built chamber. The 3D vessel lumen geometries were reconstructed manually to assess growth. Blood flow velocity was computed from the central B-scan using red blood cell particle image velocimetry. The use of extended OCT imaging as a non-invasive method for continuous and simultaneous flow and structural data can enhance our understanding of the biomechanical regulation of critical events in morphogenesis. Data acquired will be useful to validate predictive finite-element 3D growth models..

Original languageEnglish
Title of host publicationOptical Methods in Developmental Biology II
PublisherSPIE
ISBN (Print)9780819498663
DOIs
Publication statusPublished - 2014
EventOptical Methods in Developmental Biology II - San Francisco, CA, United States
Duration: 2014 Feb 12014 Feb 2

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8953
ISSN (Print)1605-7422

Other

OtherOptical Methods in Developmental Biology II
Country/TerritoryUnited States
CitySan Francisco, CA
Period14-02-0114-02-02

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials

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