Simultaneous topography and elasticity measurement of live PC-12 cells by using amplitude-modulation atomic force microscopy

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Abstract

Governing equation for describing the probe-dynamics of amplitude-modulation AFM and the mechanism of probe-to-sample interaction were formulated to develop a method for analyzing the elasticity of soft samples. This method features in combining the harmonic oscillation of the tapping probe, the surface energy, and the contact mechanics of the probe-to-sample interface with Derjaguin- Muller- Toporov model. Six PC-12 neuron-like cells were employed to test applicability of the method. The experiment results revealed that the Young's moduli of the scanned cells are consistent with the literature in order of magnitude. Using the proposed method, we could obtain the topography and global distribution of elasticity of live cells in just one scan which is more efficient than the local indentation-test with AFM.

Original languageEnglish
Title of host publication6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Pages1079-1082
Number of pages4
DOIs
Publication statusPublished - 2010 Oct 22
Event6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech - Singapore, Singapore
Duration: 2010 Aug 12010 Aug 6

Publication series

NameIFMBE Proceedings
Volume31 IFMBE
ISSN (Print)1680-0737

Other

Other6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
CountrySingapore
CitySingapore
Period10-08-0110-08-06

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

Cite this

Liu, M. C., Tien, S., Lin, C. C. K., & Ju, M. S. (2010). Simultaneous topography and elasticity measurement of live PC-12 cells by using amplitude-modulation atomic force microscopy. In 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech (pp. 1079-1082). (IFMBE Proceedings; Vol. 31 IFMBE). https://doi.org/10.1007/978-3-642-14515-5_274