TY - GEN
T1 - Simultaneous topography and elasticity measurement of live PC-12 cells by using amplitude-modulation atomic force microscopy
AU - Liu, M. C.
AU - Tien, S.
AU - Lin, C. C.K.
AU - Ju, M. S.
PY - 2010
Y1 - 2010
N2 - Governing equation for describing the probe-dynamics of amplitude-modulation AFM and the mechanism of probe-to-sample interaction were formulated to develop a method for analyzing the elasticity of soft samples. This method features in combining the harmonic oscillation of the tapping probe, the surface energy, and the contact mechanics of the probe-to-sample interface with Derjaguin- Muller- Toporov model. Six PC-12 neuron-like cells were employed to test applicability of the method. The experiment results revealed that the Young's moduli of the scanned cells are consistent with the literature in order of magnitude. Using the proposed method, we could obtain the topography and global distribution of elasticity of live cells in just one scan which is more efficient than the local indentation-test with AFM.
AB - Governing equation for describing the probe-dynamics of amplitude-modulation AFM and the mechanism of probe-to-sample interaction were formulated to develop a method for analyzing the elasticity of soft samples. This method features in combining the harmonic oscillation of the tapping probe, the surface energy, and the contact mechanics of the probe-to-sample interface with Derjaguin- Muller- Toporov model. Six PC-12 neuron-like cells were employed to test applicability of the method. The experiment results revealed that the Young's moduli of the scanned cells are consistent with the literature in order of magnitude. Using the proposed method, we could obtain the topography and global distribution of elasticity of live cells in just one scan which is more efficient than the local indentation-test with AFM.
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U2 - 10.1007/978-3-642-14515-5_274
DO - 10.1007/978-3-642-14515-5_274
M3 - Conference contribution
AN - SCOPUS:77958005829
SN - 9783540790389
T3 - IFMBE Proceedings
SP - 1079
EP - 1082
BT - 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
T2 - 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Y2 - 1 August 2010 through 6 August 2010
ER -