Single-cell electric lysis on an electroosmotic-driven microfluidic chip with arrays of microwells

Chun Ping Jen, Tamara G. Amstislavskaya, Ya Hui Liu, Ju Hsiu Hsiao, Yu Hung Chen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. An electroosmotic-driven microfluidic chip with arrays of 30-μm-diameter microwells was developed for single-cell electric lysis in the present study. The cellular occupancy in the microwells when the applied voltage was 5 V (82.4%) was slightly higher than that at an applied voltage of 10 V (81.8%). When the applied voltage was increased to 15 V, the cellular occupancy in the microwells dropped to 64.3%. More than 50% of the occupied microwells contain individual cells. The results of electric lysis experiments at the single-cell level indicate that the cells were gradually lysed as the DC voltage of 30 V was applied; the cell was fully lysed after 25 s. Single-cell electric lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis.

Original languageEnglish
Pages (from-to)6967-6977
Number of pages11
JournalSensors (Switzerland)
Volume12
Issue number6
DOIs
Publication statusPublished - 2012 Jun 1

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Biochemistry
  • Instrumentation
  • Electrical and Electronic Engineering

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