TY - GEN
T1 - Single cell real time secretion assay using amorphous fluoropolymer microwell array
AU - Shirasaki, Y.
AU - Nakahara, A.
AU - Shimura, N.
AU - Yamagishi, M.
AU - Mizuno, J.
AU - Ohara, O.
AU - Shoji, S.
PY - 2011
Y1 - 2011
N2 - Real time assay of protein secretion from living single cells was realized by coupling total internal reflection fluorescence (TIRF) microscopy observation and an optically optimized microwell array device. This system was based on a sandwich immunosorbent assay. Individual cells were cultured on an inverted microscope in microwell arrays fabricated of an amorphous fluoropolymer, CYTOP, to be monitored by TIRF imaging. CYTOP have no interference for the TIRF imaging because the refractive index of CYTOP is almost same as that of water. Using this system, we could succeed in real time secretion assay of lipopolysaccharide stimulated mast cells and the secretion time course of IL-1β from a single MC/9 cell was obtained successfully.
AB - Real time assay of protein secretion from living single cells was realized by coupling total internal reflection fluorescence (TIRF) microscopy observation and an optically optimized microwell array device. This system was based on a sandwich immunosorbent assay. Individual cells were cultured on an inverted microscope in microwell arrays fabricated of an amorphous fluoropolymer, CYTOP, to be monitored by TIRF imaging. CYTOP have no interference for the TIRF imaging because the refractive index of CYTOP is almost same as that of water. Using this system, we could succeed in real time secretion assay of lipopolysaccharide stimulated mast cells and the secretion time course of IL-1β from a single MC/9 cell was obtained successfully.
UR - http://www.scopus.com/inward/record.url?scp=80052135055&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052135055&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2011.5969330
DO - 10.1109/TRANSDUCERS.2011.5969330
M3 - Conference contribution
AN - SCOPUS:80052135055
SN - 9781457701573
T3 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
SP - 755
EP - 758
BT - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
T2 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Y2 - 5 June 2011 through 9 June 2011
ER -