Site-directed mutagenesis of Asp280 suggests substrate-assisted catalysis of chitinase Al from Bacillus circulans WL-12

Masayuki Hashimoto, Yuji Honda, Naoki Nikaidou, Tamo Fukamizo, Takeshi Watanabe

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To study the involvement of Asp280 of chitinase A1 from Bacillus circulans WL-12 in the catalytic reaction of the enzyme, site-directed mutagenesis of this residue was carried out. Kinetic analysis of the mutant chitinases revealed that the residue is not absolutely essential for the catalytic reaction and thus, does not act as the catalytic nucleophile.

Original languageEnglish
Pages (from-to)100-102
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume89
Issue number1
Publication statusPublished - 2000 Jan 1

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Chitinases
Mutagenesis
Nucleophiles
Bacilli
Site-Directed Mutagenesis
Catalysis
Bacillus
Enzymes
Kinetics
Substrates

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "To study the involvement of Asp280 of chitinase A1 from Bacillus circulans WL-12 in the catalytic reaction of the enzyme, site-directed mutagenesis of this residue was carried out. Kinetic analysis of the mutant chitinases revealed that the residue is not absolutely essential for the catalytic reaction and thus, does not act as the catalytic nucleophile.",
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Site-directed mutagenesis of Asp280 suggests substrate-assisted catalysis of chitinase Al from Bacillus circulans WL-12. / Hashimoto, Masayuki; Honda, Yuji; Nikaidou, Naoki; Fukamizo, Tamo; Watanabe, Takeshi.

In: Journal of Bioscience and Bioengineering, Vol. 89, No. 1, 01.01.2000, p. 100-102.

Research output: Contribution to journalArticle

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AU - Hashimoto, Masayuki

AU - Honda, Yuji

AU - Nikaidou, Naoki

AU - Fukamizo, Tamo

AU - Watanabe, Takeshi

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Y1 - 2000/1/1

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