Site-directed mutagenesis of Asp280 suggests substrate-assisted catalysis of chitinase Al from Bacillus circulans WL-12

Masayuki Hashimoto, Yuji Honda, Naoki Nikaidou, Tamo Fukamizo, Takeshi Watanabe

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

To study the involvement of Asp280 of chitinase A1 from Bacillus circulans WL-12 in the catalytic reaction of the enzyme, site-directed mutagenesis of this residue was carried out. Kinetic analysis of the mutant chitinases revealed that the residue is not absolutely essential for the catalytic reaction and thus, does not act as the catalytic nucleophile.

Original languageEnglish
Pages (from-to)100-102
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume89
Issue number1
DOIs
Publication statusPublished - 2000 Jan 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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