@article{c6766a4209f14dbab641fa8e597f378b,
title = "SMRTER, a Drosophila nuclear receptor coregulator, reveals that EcR-mediated repression is critical for development",
abstract = "The Drosophila ecdysone receptor (EcR)/ultraspiracle (USP) heterodimer is a key regulator in molting and metamorphoric processes, activating and repressing transcription in a sequence-specific manner. Here, we report the isolation of an EcR-interacting protein, SMRTER, which is structurally divergent but functionally similar to the vertebrate nuclear corepressors SMRT and N-CoR. SMRTER mediates repression by interacting with Sin3A, a repressor known to form a complex with the histone deacetylase Rpd3/HDAC. Importantly, we identify an EcR mutant allele that fails to bind SMRTER and is characterized by developmental defects and lethality. Together, these results reveal a novel nuclear receptor cofactor that exhibits evolutionary conservation in the mechanism to achieve repression and demonstrate the essential role of repression in hormone signaling.",
author = "Tsai, {Chih Cheng} and Kao, {Hung Ying} and Yao, {Tso Pang} and Michael McKeown and Evans, {Ronald M.}",
note = "Funding Information: We especially thank A. Tang and G. Rubin for the dSin3A c-DNA and dSin3A mutants prior to their publication; D. Chen for examining the interaction between EcR and SMRT; M. Bender, F. Kafatos, S. Elledge, L. Pick, T. Hazelrigg, J. Tamkun, and colleagues in the lab for making their reagents available; R. Head and P. Ordentlich for comments on the manuscript; Lita Ong and Elaine Stevens for administrative assistance. We acknowledge E. Banayo, S. Trieu, E. White, and H. Juguilon for their technical assistance. C.-C. T. is a fellow of the Damon Runyon-Walter Winchell Cancer Fund (DRG 1366). H.-Y. K. is supported by the Leukemia Society of America. M. M. is an investigator of the Salk Institute. R. M. E. is an investigator of the Howard Hughes Medical Institute at the Salk Institute for Biological Studies and March of Dimes Chair in Molecular and Developmental Biology. This work was supported by the Howard Hughes Medical Institute, NIH grants HD27183 and GM26444, the Mathers Foundation, and the Joe and Dorothy Brown Dorsett Foundation. ",
year = "1999",
month = aug,
doi = "10.1016/S1097-2765(00)80365-2",
language = "English",
volume = "4",
pages = "175--186",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "2",
}