Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1

Takahisa Ikegami, Terumasa Okada, Masayuki Hashimoto, Shizuka Seino, Takeshi Watanabe, Masahiro Shirakawa

Research output: Contribution to journalArticle

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Abstract

The three-dimensional structure of the chitin-binding domain (ChBD) of chitinase A1 (ChiA1) from a Gram-positive bacterium, Bacillus circulans WL- 12, was determined by means of multidimensional heteronuclear NMR methods. ChiA1 is a glycosidase that hydrolyzes chitin and is composed of an N- terminal catalytic domain, two fibronectin type III-like domains, and C- terminal ChBD(chiA1) (45 residues, Ala655-Gln699), which binds specifically to insoluble chitin. ChBD(ChiA1) has a compact and globular structure with the topology of a twisted β-sandwich. This domain contains two antiparallel β-sheets, one composed of three strands and the other of two strands. The core region formed by the hydrophobic and aromatic residues makes the overall structure rigid and compact. The overall topology of ChBD(ChiA1) is similar to that of the cellulose-binding domain (CBD) of Erwinia chrysanthemi endoglucanase Z (CBD(EGZ)). However, ChBD(ChiA1) lacks the three aromatic residues aligned linearly and exposed to the solvent, which probably interact with cellulose in CBDs. Therefore, the binding mechanism of a group of ChBDs including ChBD(ChiA1) may be different from that proposed for CBDs.

Original languageEnglish
Pages (from-to)13654-13661
Number of pages8
JournalJournal of Biological Chemistry
Volume275
Issue number18
DOIs
Publication statusPublished - 2000 May 5

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Chitinases
Chitin
Bacilli
Bacillus
Cellulose
Pectobacterium chrysanthemi
Topology
Biomolecular Nuclear Magnetic Resonance
Rigid structures
Glycoside Hydrolases
Gram-Positive Bacteria
Fibronectins
Catalytic Domain
Bacteria
Nuclear magnetic resonance

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ikegami, Takahisa ; Okada, Terumasa ; Hashimoto, Masayuki ; Seino, Shizuka ; Watanabe, Takeshi ; Shirakawa, Masahiro. / Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 18. pp. 13654-13661.
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Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1. / Ikegami, Takahisa; Okada, Terumasa; Hashimoto, Masayuki; Seino, Shizuka; Watanabe, Takeshi; Shirakawa, Masahiro.

In: Journal of Biological Chemistry, Vol. 275, No. 18, 05.05.2000, p. 13654-13661.

Research output: Contribution to journalArticle

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