Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a)

Pei Phen Liu, Yen Chin Chen, Ching Li, Yu Huei Hsieh, Shu Wan Chen, Shu Huei Chen, Wen Yih Jeng, Woei Jer Chuang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Interleukin enhancer binding factor (ILF) binds to the interleukin-2 (IL-2) promoter and regulates IL-2 gene expression. In this study, the 3D structure of the DNA-binding domain of ILF was determined by multidimensional NMR spectroscopy. NMR structure analysis revealed that the DNA-binding domain of ILF is a new member of the winged helix/forkhead family, and that its wing 2 contains an extra α-helix. This is the first study to report the presence of a C-terminal α-helix in place of a typical wing 2 in a member of this family. This structural difference may be responsible for the different DNA-binding specificity of ILF compared to other winged helix/forkhead proteins. Our deletion studies of the fragments of ILF also suggest that the C-terminal region plays a regulatory role in DNA binding.

Original languageEnglish
Pages (from-to)543-553
Number of pages11
JournalProteins: Structure, Function and Genetics
Volume49
Issue number4
DOIs
Publication statusPublished - 2002 Dec 1

Fingerprint

Interleukins
DNA
Interleukin-2
Forkhead Transcription Factors
Gene expression
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Gene Expression
interleukin binding factor

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

Liu, Pei Phen ; Chen, Yen Chin ; Li, Ching ; Hsieh, Yu Huei ; Chen, Shu Wan ; Chen, Shu Huei ; Jeng, Wen Yih ; Chuang, Woei Jer. / Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a). In: Proteins: Structure, Function and Genetics. 2002 ; Vol. 49, No. 4. pp. 543-553.
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Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a). / Liu, Pei Phen; Chen, Yen Chin; Li, Ching; Hsieh, Yu Huei; Chen, Shu Wan; Chen, Shu Huei; Jeng, Wen Yih; Chuang, Woei Jer.

In: Proteins: Structure, Function and Genetics, Vol. 49, No. 4, 01.12.2002, p. 543-553.

Research output: Contribution to journalArticle

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AU - Liu, Pei Phen

AU - Chen, Yen Chin

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AU - Chen, Shu Wan

AU - Chen, Shu Huei

AU - Jeng, Wen Yih

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