Solution structure of the fibronectin type III domain from Bacillus circulans WL-12 chitinase A1

Jun Goo Jee, Takahisa Ikegami, Masayuki Hashimoto, Takeshi Kawabata, Mitsunori Ikeguchi, Takeshi Watanabe, Masahiro Shirakawa

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Growing evidence suggests that horizontal gene transfer plays an integral role in the evolution of bacterial genomes. One of the debated examples of horizontal gene transfer from animal to prokaryote is the fibronectin type III domain (FnIIID). Certain extracellular proteins of soil bacteria contain an unusual cluster of Fn-IIIDs, which show sequence similarity to those of animals and are likely to have been acquired horizontally from animals. Here we report the solution structure of the FnIIID of chitinase A1 from Bacillus circulans WL-12. To the best of our knowledge, this is the first tertiary structure to be reported for an FnIIID from a bacterial protein. The structure of the domain shows significant similarity to FnIIIDs from animal proteins. Sequence comparisons with FnIIIDs from other soil bacteria proteins show that the core-forming residues are highly conserved and, thus, are under strong evolutionary pressure. Striking similarities in the tertiary structures of bacterial FnIIIDs and their mammalian counterparts may support the hypothesis that the evolution of the FnIIID in bacterial carbohydrases occurred horizontally. The total lack of surface-exposed aromatic residues also suggests that the role of this FnIIID is different from those of other bacterial β-sandwich domains, which function as carbohydrate-binding modules.

Original languageEnglish
Pages (from-to)1388-1397
Number of pages10
JournalJournal of Biological Chemistry
Volume277
Issue number2
DOIs
Publication statusPublished - 2002 Jan 11

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Chitinases
Bacilli
Fibronectins
Bacillus
Animals
Gene transfer
Horizontal Gene Transfer
Bacteria
Soil
Bacterial Genomes
Bacterial Structures
Soils
Proteins
Bacterial Proteins
Genes
Carbohydrates
Fibronectin Type III Domain
Pressure

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Jee, Jun Goo ; Ikegami, Takahisa ; Hashimoto, Masayuki ; Kawabata, Takeshi ; Ikeguchi, Mitsunori ; Watanabe, Takeshi ; Shirakawa, Masahiro. / Solution structure of the fibronectin type III domain from Bacillus circulans WL-12 chitinase A1. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 2. pp. 1388-1397.
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Solution structure of the fibronectin type III domain from Bacillus circulans WL-12 chitinase A1. / Jee, Jun Goo; Ikegami, Takahisa; Hashimoto, Masayuki; Kawabata, Takeshi; Ikeguchi, Mitsunori; Watanabe, Takeshi; Shirakawa, Masahiro.

In: Journal of Biological Chemistry, Vol. 277, No. 2, 11.01.2002, p. 1388-1397.

Research output: Contribution to journalArticle

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