Glued laminated timber, also called Glulam, was applied on various building components, space products, and decoration materials. The extensively application of Glulam was attributed to its feature with durable, ease processing, attractive appearance, and sustainability. According to the feature of Glulam, in present the Glulam was frequently used to combine with multiple materials such as metal, gypsum panel, rubber, and even paper materials. The composite wood panel with multiple materials would be effective to fire prevention and sound insulation. However, the analysis of sound insulation of composite wood panel was still focused on the surface density but no more controlling factors of sound insulation for quantitative analysis. By the new technique of composite wood panel, the uncertain of quality analysis would cost wasting from repeatedly replacement during construction just because of the ineffective sound insulation analysis. This study was focused on Glulam. The composite wood panel would be combined with different material. Furthermore, it was confirmed for the influence of sound insulation with various factors, such as the location of material, rigidity, and simple mechanism. The raising surface density for the material near the median of panel section would promote the sound insulation between 500 Hz to 2000 Hz with about 5 dB. In the other side, the raising rigidity and resonance frequency for the material near the surface of panelto 7 dB. Above this study, it could extend to more analysis for composite wood panel, and would make more effective accuracy for sound insulation analysis.