TY - JOUR
T1 - Spatially reinforced Au nano-cavities as a reaction nano-reservoir for trace analysis of DNA hybridization
AU - Yao, Chih Kai
AU - Liao, Jiunn Der
AU - Lin, Chih Heng
AU - Yang, Yuh Shyong
AU - Yu, Sheng Hong
AU - Yang, Jung Wei
N1 - Funding Information:
This work was financially supported by National Science Council of Taiwan under Grant No. NSC-100-2221-E-006-025-MY3 and Medical Device Innovation Center of National Cheng Kung University under the Grant No. D102-21007 , and Headquarters of University Advancement, NCKU , under the Grant No. D102-33B08 .
PY - 2014
Y1 - 2014
N2 - Spatially reinforced Au nano-cavities (SR-nAu) with reduced tip-to-tip displacement create strongly localized surface plasmon resonance by Raman scattering. Within SR-nAu, the nano-cavity structure with high aspect ratio was optimized as the nano-reservoir for the distinction of DNA hybridization responses from specific DNA sequences of Avian influenza virus. In the nano-reservoir, the suspended or immobilized probe, its complementary and noncomplementary target sequences were characterized at very low concentration through enhanced Raman spectra. The presence of Raman shift at 738 cm -1, the characteristic breathing mode of thymine, and the change of relative peaks intensity at 1603 and 1554 cm-1 before and after hybridization were particularly taken as the major indications to validate the match or mismatch state of DNA sequences. Through a labeling-free means, and from the evidence provided by specific complementary and noncomplementary DNA sequences, the recognition capability of nano-reservoir can be down to subnano-molar concentration (10-10 M).
AB - Spatially reinforced Au nano-cavities (SR-nAu) with reduced tip-to-tip displacement create strongly localized surface plasmon resonance by Raman scattering. Within SR-nAu, the nano-cavity structure with high aspect ratio was optimized as the nano-reservoir for the distinction of DNA hybridization responses from specific DNA sequences of Avian influenza virus. In the nano-reservoir, the suspended or immobilized probe, its complementary and noncomplementary target sequences were characterized at very low concentration through enhanced Raman spectra. The presence of Raman shift at 738 cm -1, the characteristic breathing mode of thymine, and the change of relative peaks intensity at 1603 and 1554 cm-1 before and after hybridization were particularly taken as the major indications to validate the match or mismatch state of DNA sequences. Through a labeling-free means, and from the evidence provided by specific complementary and noncomplementary DNA sequences, the recognition capability of nano-reservoir can be down to subnano-molar concentration (10-10 M).
UR - http://www.scopus.com/inward/record.url?scp=84886995299&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886995299&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2013.09.120
DO - 10.1016/j.snb.2013.09.120
M3 - Article
AN - SCOPUS:84886995299
SN - 0925-4005
VL - 191
SP - 219
EP - 226
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -