@inproceedings{a4c811f8f86247f5bc33aed6d5f40368,
title = "Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning of thick tissues",
abstract = "Conventional multiphoton microscopy employs beam scanning; however, in this study a microscope based on spatiotemporal focusing offering widefield multiphoton excitation has been developed to provide fast optical sectioning images. The microscope integrates a 10 kHz repetition rate ultrafast amplifier featuring strong instantaneous peak power (maximum 400 μJ/pulse at 90 fs pulse width) with a TE-cooled, ultra-sensitive photon detecting, electron multiplying charge-coupled device camera. This configuration can produce multiphoton excited images with an excitation area larger than 200 × 100 μm2 at a frame rate greater than 100 Hz. Brownian motions of fluorescent microbeads as small as 0.5 μm have been instantaneously observed with a lateral spatial resolution of less than 0.5 μm and an axial resolution of approximately 3.5 μm. Moreover, we combine the widefield multiphoton microscopy with structure illuminated technique named HiLo to reject the background scattering noise to get better quality for bioimaging.",
author = "Cheng, {Li Chung} and Chang, {Chia Yuan} and Yen, {Wei Chung} and Chen, {Shean Jen}",
year = "2012",
doi = "10.1117/12.930324",
language = "English",
isbn = "9780819492371",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
booktitle = "Unconventional Imaging and Wavefront Sensing 2012",
note = "Unconventional Imaging and Wavefront Sensing 2012 ; Conference date: 13-08-2012 Through 14-08-2012",
}