Spawning stress triggers WSSV replication in brooders via the activation of shrimp STAT

Shin Jen Lin, Hui Lan Hsia, Wang Jing Liu, Jiun Yan Huang, Kuan Fu Liu, Wei Yu Chen, Ying Chun Yeh, Yun Tzu Huang, Chu Fang Lo, Guang Hsiung Kou, Han Ching Wang

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

In the early days of shrimp aquaculture, wild-captured brooders usually spawned repeatedly once every 2-4. days. However, since the first outbreaks of white spot disease (WSD) nearly 20. years ago, captured female brooders often died soon after a single spawning. Although these deaths were clearly attributable to WSD, it has always been unclear how spawning stress could lead to an outbreak of the disease. Using real-time qPCR, we show here that while replication of the white spot syndrome virus (WSSV; the causative agent of WSD) is triggered by spawning, there was no such increase in the levels of another shrimp DNA virus, IHHNV (infectious hypodermal and hematopoietic necrosis virus). We also show that levels of activated STAT are increased in brooders during and after spawning, which is important because shrimp STAT is known to transactivate the expression of the WSSV immediate early gene . ie1. Lastly, we used dsRNA silencing experiment to show that both WSSV . ie1 gene expression and WSSV genome copy number were reduced significantly after shrimp . STAT was knocked-down. This is the first report to demonstrate . in vivo that shrimp STAT is important for WSSV replication and that spawning stress increases activated STAT, which in turn triggers WSSV replication in WSSV-infected brooders.

Original languageEnglish
Pages (from-to)128-135
Number of pages8
JournalDevelopmental and Comparative Immunology
Volume38
Issue number1
DOIs
Publication statusPublished - 2012 Sep 1

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

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