Abstract
We report that human mesenchymal stem cells (hMSCs) were successfully labeled with poly(lactideco-glycolide) nanoparticles (PLGA NPs) surface-conjugated quantum dots (QDs) (PLGA-QD NPs) via endocytosis pathway. These NPs were not toxicity even treated with PLGA-QD NPs at high concentrations for at least four weeks. Besides, PLGA-QD NPs-labeled hMSCs did not change their proliferation and differentiation capability toward the cell fates of adipocytes, osteocytes, and chrondrocytes. It's known that PLGA has been widely employed to act as delivery carrier which encapsulates drugs and releases them under a controlled way. Currently, we have also demonstrated that FITC-loaded PLGA-QD NPs degraded in hMSCs to achieve intracellular release of FITC. The aim of this research is to investigate viability, proliferation and differentiation capability and the potential for gene delivery of MSCs labeled with PLGA-QD NPs. In addition to PLGA-QD NPs, QDs alone were used to serve as a control set for comparison.
| Original language | English |
|---|---|
| Pages (from-to) | 940-948 |
| Number of pages | 9 |
| Journal | Journal of the Chinese Chemical Society |
| Volume | 56 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2009 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
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Stabilizer-free poly(lactide-co-glycolide) nanoparticles conjugated with quantum dots as a potential carrier applied in human mesenchymal stem cells. / Kuo, Wen Shuo; Hwang, Shiaw Min; Sei, Hei Tin; Ku, Yu Cian; Hsu, Lee Feng; Cheng, Fong Yu; Hsieh, Patrick Ching Ho; Yeh, Chen-Sheng.
In: Journal of the Chinese Chemical Society, Vol. 56, No. 5, 01.01.2009, p. 940-948.Research output: Contribution to journal › Article
TY - JOUR
T1 - Stabilizer-free poly(lactide-co-glycolide) nanoparticles conjugated with quantum dots as a potential carrier applied in human mesenchymal stem cells
AU - Kuo, Wen Shuo
AU - Hwang, Shiaw Min
AU - Sei, Hei Tin
AU - Ku, Yu Cian
AU - Hsu, Lee Feng
AU - Cheng, Fong Yu
AU - Hsieh, Patrick Ching Ho
AU - Yeh, Chen-Sheng
PY - 2009/1/1
Y1 - 2009/1/1
N2 - We report that human mesenchymal stem cells (hMSCs) were successfully labeled with poly(lactideco-glycolide) nanoparticles (PLGA NPs) surface-conjugated quantum dots (QDs) (PLGA-QD NPs) via endocytosis pathway. These NPs were not toxicity even treated with PLGA-QD NPs at high concentrations for at least four weeks. Besides, PLGA-QD NPs-labeled hMSCs did not change their proliferation and differentiation capability toward the cell fates of adipocytes, osteocytes, and chrondrocytes. It's known that PLGA has been widely employed to act as delivery carrier which encapsulates drugs and releases them under a controlled way. Currently, we have also demonstrated that FITC-loaded PLGA-QD NPs degraded in hMSCs to achieve intracellular release of FITC. The aim of this research is to investigate viability, proliferation and differentiation capability and the potential for gene delivery of MSCs labeled with PLGA-QD NPs. In addition to PLGA-QD NPs, QDs alone were used to serve as a control set for comparison.
AB - We report that human mesenchymal stem cells (hMSCs) were successfully labeled with poly(lactideco-glycolide) nanoparticles (PLGA NPs) surface-conjugated quantum dots (QDs) (PLGA-QD NPs) via endocytosis pathway. These NPs were not toxicity even treated with PLGA-QD NPs at high concentrations for at least four weeks. Besides, PLGA-QD NPs-labeled hMSCs did not change their proliferation and differentiation capability toward the cell fates of adipocytes, osteocytes, and chrondrocytes. It's known that PLGA has been widely employed to act as delivery carrier which encapsulates drugs and releases them under a controlled way. Currently, we have also demonstrated that FITC-loaded PLGA-QD NPs degraded in hMSCs to achieve intracellular release of FITC. The aim of this research is to investigate viability, proliferation and differentiation capability and the potential for gene delivery of MSCs labeled with PLGA-QD NPs. In addition to PLGA-QD NPs, QDs alone were used to serve as a control set for comparison.
UR - http://www.scopus.com/inward/record.url?scp=77952378681&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77952378681&partnerID=8YFLogxK
U2 - 10.1002/jccs.200900138
DO - 10.1002/jccs.200900138
M3 - Article
AN - SCOPUS:77952378681
VL - 56
SP - 940
EP - 948
JO - Journal of the Chinese Chemical Society
JF - Journal of the Chinese Chemical Society
SN - 0009-4536
IS - 5
ER -