Stabilizer-free poly(lactide-co-glycolide) nanoparticles conjugated with quantum dots as a potential carrier applied in human mesenchymal stem cells

Wen Shuo Kuo, Shiaw Min Hwang, Hei Tin Sei, Yu Cian Ku, Lee Feng Hsu, Fong Yu Cheng, Patrick Ching Ho Hsieh, Chen-Sheng Yeh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We report that human mesenchymal stem cells (hMSCs) were successfully labeled with poly(lactideco-glycolide) nanoparticles (PLGA NPs) surface-conjugated quantum dots (QDs) (PLGA-QD NPs) via endocytosis pathway. These NPs were not toxicity even treated with PLGA-QD NPs at high concentrations for at least four weeks. Besides, PLGA-QD NPs-labeled hMSCs did not change their proliferation and differentiation capability toward the cell fates of adipocytes, osteocytes, and chrondrocytes. It's known that PLGA has been widely employed to act as delivery carrier which encapsulates drugs and releases them under a controlled way. Currently, we have also demonstrated that FITC-loaded PLGA-QD NPs degraded in hMSCs to achieve intracellular release of FITC. The aim of this research is to investigate viability, proliferation and differentiation capability and the potential for gene delivery of MSCs labeled with PLGA-QD NPs. In addition to PLGA-QD NPs, QDs alone were used to serve as a control set for comparison.

Original languageEnglish
Pages (from-to)940-948
Number of pages9
JournalJournal of the Chinese Chemical Society
Volume56
Issue number5
DOIs
Publication statusPublished - 2009 Jan 1

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Polyglactin 910
Stem cells
Semiconductor quantum dots
Nanoparticles
Fluorescein-5-isothiocyanate
polylactic acid-polyglycolic acid copolymer
Toxicity
Genes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Kuo, Wen Shuo ; Hwang, Shiaw Min ; Sei, Hei Tin ; Ku, Yu Cian ; Hsu, Lee Feng ; Cheng, Fong Yu ; Hsieh, Patrick Ching Ho ; Yeh, Chen-Sheng. / Stabilizer-free poly(lactide-co-glycolide) nanoparticles conjugated with quantum dots as a potential carrier applied in human mesenchymal stem cells. In: Journal of the Chinese Chemical Society. 2009 ; Vol. 56, No. 5. pp. 940-948.
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title = "Stabilizer-free poly(lactide-co-glycolide) nanoparticles conjugated with quantum dots as a potential carrier applied in human mesenchymal stem cells",
abstract = "We report that human mesenchymal stem cells (hMSCs) were successfully labeled with poly(lactideco-glycolide) nanoparticles (PLGA NPs) surface-conjugated quantum dots (QDs) (PLGA-QD NPs) via endocytosis pathway. These NPs were not toxicity even treated with PLGA-QD NPs at high concentrations for at least four weeks. Besides, PLGA-QD NPs-labeled hMSCs did not change their proliferation and differentiation capability toward the cell fates of adipocytes, osteocytes, and chrondrocytes. It's known that PLGA has been widely employed to act as delivery carrier which encapsulates drugs and releases them under a controlled way. Currently, we have also demonstrated that FITC-loaded PLGA-QD NPs degraded in hMSCs to achieve intracellular release of FITC. The aim of this research is to investigate viability, proliferation and differentiation capability and the potential for gene delivery of MSCs labeled with PLGA-QD NPs. In addition to PLGA-QD NPs, QDs alone were used to serve as a control set for comparison.",
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Stabilizer-free poly(lactide-co-glycolide) nanoparticles conjugated with quantum dots as a potential carrier applied in human mesenchymal stem cells. / Kuo, Wen Shuo; Hwang, Shiaw Min; Sei, Hei Tin; Ku, Yu Cian; Hsu, Lee Feng; Cheng, Fong Yu; Hsieh, Patrick Ching Ho; Yeh, Chen-Sheng.

In: Journal of the Chinese Chemical Society, Vol. 56, No. 5, 01.01.2009, p. 940-948.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stabilizer-free poly(lactide-co-glycolide) nanoparticles conjugated with quantum dots as a potential carrier applied in human mesenchymal stem cells

AU - Kuo, Wen Shuo

AU - Hwang, Shiaw Min

AU - Sei, Hei Tin

AU - Ku, Yu Cian

AU - Hsu, Lee Feng

AU - Cheng, Fong Yu

AU - Hsieh, Patrick Ching Ho

AU - Yeh, Chen-Sheng

PY - 2009/1/1

Y1 - 2009/1/1

N2 - We report that human mesenchymal stem cells (hMSCs) were successfully labeled with poly(lactideco-glycolide) nanoparticles (PLGA NPs) surface-conjugated quantum dots (QDs) (PLGA-QD NPs) via endocytosis pathway. These NPs were not toxicity even treated with PLGA-QD NPs at high concentrations for at least four weeks. Besides, PLGA-QD NPs-labeled hMSCs did not change their proliferation and differentiation capability toward the cell fates of adipocytes, osteocytes, and chrondrocytes. It's known that PLGA has been widely employed to act as delivery carrier which encapsulates drugs and releases them under a controlled way. Currently, we have also demonstrated that FITC-loaded PLGA-QD NPs degraded in hMSCs to achieve intracellular release of FITC. The aim of this research is to investigate viability, proliferation and differentiation capability and the potential for gene delivery of MSCs labeled with PLGA-QD NPs. In addition to PLGA-QD NPs, QDs alone were used to serve as a control set for comparison.

AB - We report that human mesenchymal stem cells (hMSCs) were successfully labeled with poly(lactideco-glycolide) nanoparticles (PLGA NPs) surface-conjugated quantum dots (QDs) (PLGA-QD NPs) via endocytosis pathway. These NPs were not toxicity even treated with PLGA-QD NPs at high concentrations for at least four weeks. Besides, PLGA-QD NPs-labeled hMSCs did not change their proliferation and differentiation capability toward the cell fates of adipocytes, osteocytes, and chrondrocytes. It's known that PLGA has been widely employed to act as delivery carrier which encapsulates drugs and releases them under a controlled way. Currently, we have also demonstrated that FITC-loaded PLGA-QD NPs degraded in hMSCs to achieve intracellular release of FITC. The aim of this research is to investigate viability, proliferation and differentiation capability and the potential for gene delivery of MSCs labeled with PLGA-QD NPs. In addition to PLGA-QD NPs, QDs alone were used to serve as a control set for comparison.

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