Stable-isotope reductive dimethylation, a costeffective, simple, robust, reliable and easy-tomultiplex labelling method, is widely applied to quantitative proteomics using liquid chromatography-mass spectrometry. This review focuses on biological applications of stable-isotope dimethyl labelling for a large-scale comparative analysis of protein expression and post-translational modifications based on its unique properties of the labelling chemistry. Some other applications of the labelling method for sample preparation and mass spectrometry-based protein identification and characterization are also summarized.
|Journal||Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences|
|Publication status||Published - 2016 Oct 28|
All Science Journal Classification (ASJC) codes
- Physics and Astronomy(all)