Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis

P. Y. Lai, Chia-Yih Wang, W. Y. Chen, Y. H. Kao, H. M. Tsai, T. Tachibana, W. C. Chang, B. C. Chung

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

SF-1 (Steroidogenic Factor 1, NR5A1) is a tissue-specific transcription factor critical for the growth, development and differentiation of steroidogenic and a few other endocrine tissues. But how SF-1 regulates cell growth is not entirely clear. Here we found that SF-1 was localized to the centrosome in addition to the nucleus, and SF-1 depletion by shRNA caused centrosome over-duplication, aberrant mitosis and genomic instability, leading to a reduction of cell number. Centrosome amplification defect was rescued by both wild-type SF-1 and transcription-defective SF-1-G35E, suggesting a non-genomic activity of SF-1 involved in centrosome homeostasis. In addition, we identified in SF-1 a centrosome localization signal, whose overexpression led to reduced localization of both SF-1 and γ-tubulin to the centrosome. Our results uncover a novel role of SF-1 in the control of centrosome homeostasis and genomic stability.

Original languageEnglish
Pages (from-to)1836-1844
Number of pages9
JournalCell Death and Differentiation
Volume18
Issue number12
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

Steroidogenic Factor 1
Centrosome
Genomic Instability
Homeostasis
Tubulin
Growth and Development
Mitosis
Small Interfering RNA
Transcription Factors
Cell Count

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Lai, P. Y. ; Wang, Chia-Yih ; Chen, W. Y. ; Kao, Y. H. ; Tsai, H. M. ; Tachibana, T. ; Chang, W. C. ; Chung, B. C. / Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis. In: Cell Death and Differentiation. 2011 ; Vol. 18, No. 12. pp. 1836-1844.
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Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis. / Lai, P. Y.; Wang, Chia-Yih; Chen, W. Y.; Kao, Y. H.; Tsai, H. M.; Tachibana, T.; Chang, W. C.; Chung, B. C.

In: Cell Death and Differentiation, Vol. 18, No. 12, 01.12.2011, p. 1836-1844.

Research output: Contribution to journalArticle

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