Streptococcal collagen-like surface protein 1 promotes adhesion to the respiratory epithelial cell.

Shih Ming Chen, Yau-Sheng Tsai, Chin Ming Wu, Shuen Kuei Liao, Ling Chia Wu, Cherng Shyang Chang, Ya Hui Liu, Pei-Jane Tsai

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Collagen-like surface proteins Scl1 and Scl2 on Streptococcus pyogenes contain contiguous Gly-X-X triplet amino acid motifs, the characteristic structure of human collagen. Although the potential role of Scl1 in adhesion has been studied, the conclusions may be affected by the use of different S. pyogenes strains and their carriages of various adhesins. To explore the bona fide nature of Scl1 in adherence to human epithelial cells without the potential interference of other streptococcal surface factors, we constructed a scl1 isogenic mutant from the Scl2-defective S. pyogenes strain and a Scl1-expressed Escherichia coli. Loss of Scl1 in a Scl2-defective S. pyogenes strain dramatically decreased the adhesion of bacteria to HEp-2 human epithelial cells. Expression of Scl1 on the surface of the heterologous bacteria E. coli significantly increased adhesion to HEp-2. The increase in adhesion was nullified when Scl1-expressed E. coli was pre-incubated with proteases or antibodies against recombinant Scl1 (rScl1) protein. Treatment of HEp-2 cells with rScl protein or pronase drastically reduced the binding capability of Scl1-expressed E. coli. These findings suggest that the adhesion is mediated through Scl1 on bacterial surface and protein receptor(s) on epithelial cells. Further blocking of potential integrins revealed significant contributions of α2 and β1 integrins in Scl1-mediated binding to epithelial cells. Together, these results underscore the importance of Scl1 in the virulence of S. pyogenes and implicate Scl1 as an adhesin during pathogenesis of streptococcal infection.

Original languageEnglish
Number of pages1
JournalBMC microbiology
Volume10
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

Streptococcus pyogenes
Membrane Proteins
Collagen
Epithelial Cells
Escherichia coli
Integrins
Bacteria
Streptococcal Infections
Pronase
Amino Acid Motifs
Bacterial Proteins
Recombinant Proteins
Virulence
Peptide Hydrolases
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Chen, Shih Ming ; Tsai, Yau-Sheng ; Wu, Chin Ming ; Liao, Shuen Kuei ; Wu, Ling Chia ; Chang, Cherng Shyang ; Liu, Ya Hui ; Tsai, Pei-Jane. / Streptococcal collagen-like surface protein 1 promotes adhesion to the respiratory epithelial cell. In: BMC microbiology. 2010 ; Vol. 10.
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Streptococcal collagen-like surface protein 1 promotes adhesion to the respiratory epithelial cell. / Chen, Shih Ming; Tsai, Yau-Sheng; Wu, Chin Ming; Liao, Shuen Kuei; Wu, Ling Chia; Chang, Cherng Shyang; Liu, Ya Hui; Tsai, Pei-Jane.

In: BMC microbiology, Vol. 10, 01.12.2010.

Research output: Contribution to journalArticle

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