Streptococcal pyrogenic exotoxin B cleaves human S-adenosylhomocysteine hydrolase and induces hypermethioninemia

Ju Fei Hsu, Woei Jer Chuang, Shu Chu Shiesh, Yee Shin Lin, Ching Chuan Liu, Chih Chieh Wang, Tzu N. Fu, Jui He Tsai, Wei Lun Tsai, Yu Jun Huang, Yi Hsuan Hsieh, Jiunn Jong Wu, Ming T. Lin, Wenya Huang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Group A Streptococcus is a common pathogen that causes pharyngitis, impetigo, myositis, and lethal streptococcal toxic shock syndrome. Streptococcal pyrogenic exotoxin B (SPE B) is strongly associated with the severity of disease. SPE B is a cysteine protease and matures itself by autocatalysis. We found that SPE B was directly associated with human S-adenosylhomocysteine hydrolase (AdoHcyase), an essential factor for a delayed-type immune response. AdoHcyase protein levels and enzymatic activities were significantly higher in human cells infected with the Streptococcus pyogenes SW510 speB mutant strain than in cells infected with the NZ131 wild-type strain. SPE B also inactivated AdoHcyase, shown by a decrease in homocysteine, the main product of AdoHcyase. We found that in vivo and in vitro, SPE B induced hypermethioninemia, which is caused by an AdoHcyase defect. We also found that AdoHcyase is a substrate of SPE B cysteine protease. SPE B, therefore, potentially causes immunosuppression by cleaving AdoHcyase.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalJournal of Infectious Diseases
Volume198
Issue number3
DOIs
Publication statusPublished - 2008 Aug 1

Fingerprint

Adenosylhomocysteinase
Cysteine Proteases
Impetigo
Myositis
Pharyngitis
Streptococcus pyogenes
Homocysteine
Septic Shock
erythrogenic toxin
Hypermethioninemia
Streptococcus
Immunosuppression

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Hsu, Ju Fei ; Chuang, Woei Jer ; Shiesh, Shu Chu ; Lin, Yee Shin ; Liu, Ching Chuan ; Wang, Chih Chieh ; Fu, Tzu N. ; Tsai, Jui He ; Tsai, Wei Lun ; Huang, Yu Jun ; Hsieh, Yi Hsuan ; Wu, Jiunn Jong ; Lin, Ming T. ; Huang, Wenya. / Streptococcal pyrogenic exotoxin B cleaves human S-adenosylhomocysteine hydrolase and induces hypermethioninemia. In: Journal of Infectious Diseases. 2008 ; Vol. 198, No. 3. pp. 367-374.
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Streptococcal pyrogenic exotoxin B cleaves human S-adenosylhomocysteine hydrolase and induces hypermethioninemia. / Hsu, Ju Fei; Chuang, Woei Jer; Shiesh, Shu Chu; Lin, Yee Shin; Liu, Ching Chuan; Wang, Chih Chieh; Fu, Tzu N.; Tsai, Jui He; Tsai, Wei Lun; Huang, Yu Jun; Hsieh, Yi Hsuan; Wu, Jiunn Jong; Lin, Ming T.; Huang, Wenya.

In: Journal of Infectious Diseases, Vol. 198, No. 3, 01.08.2008, p. 367-374.

Research output: Contribution to journalArticle

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AU - Hsu, Ju Fei

AU - Chuang, Woei Jer

AU - Shiesh, Shu Chu

AU - Lin, Yee Shin

AU - Liu, Ching Chuan

AU - Wang, Chih Chieh

AU - Fu, Tzu N.

AU - Tsai, Jui He

AU - Tsai, Wei Lun

AU - Huang, Yu Jun

AU - Hsieh, Yi Hsuan

AU - Wu, Jiunn Jong

AU - Lin, Ming T.

AU - Huang, Wenya

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