Stretch-stimulated activity of large conductance calcium-activated potassium channels in human retinal pigment epithelial cells

Shwu Jiuan Sheu, Sheng-Nan Wu, Dan Ning Hu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aim: The aim of this study was to investigate the effect of membrane stretch on the activity of BKCa channels and determine whether change in BKCa-channel activity induced by membrane stretch can be altered in the presence of riluzole. Methods: The effect and mechanisms of membrane stretch on the activity of large-conductance Ca2+-activated K + (BKCa) channels in human retinal pigment epithelial (RPE) cells were investigated using the patch-clamp technique. Results: In cell-attached patches, an increase in BKCa-channel activity was induced by membrane stretch, which is mainly mediated by the increase in the number of long-lived openings. With inside-out patches, application of pipette suction did not modify single-channel conductance of BKCa channels; however, it did enhance the channel activity. Membrane stretch-induced channel activity was found to be independent of the level of intracellular Ca 2+. Membrane stretch also potentiated riluzole-stimulated BK Ca-channel activity. Conclusions: BKCa channel can be stimulated by membrane stretch in human RPE cells. During an increase in tensile strength of RPE, the BKCa channel might exert a protective action against deleterious tension by promoting a hyperpolarizing outflow that forces the cell to rest.

Original languageEnglish
Pages (from-to)429-435
Number of pages7
JournalJournal of Ocular Pharmacology and Therapeutics
Volume21
Issue number6
DOIs
Publication statusPublished - 2005 Dec 1

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Large-Conductance Calcium-Activated Potassium Channels
Retinal Pigments
Epithelial Cells
Membranes
Riluzole
Calcium-Activated Potassium Channels
Tensile Strength
Suction
Patch-Clamp Techniques

All Science Journal Classification (ASJC) codes

  • Pharmacology (medical)
  • Ophthalmology
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

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abstract = "Aim: The aim of this study was to investigate the effect of membrane stretch on the activity of BKCa channels and determine whether change in BKCa-channel activity induced by membrane stretch can be altered in the presence of riluzole. Methods: The effect and mechanisms of membrane stretch on the activity of large-conductance Ca2+-activated K + (BKCa) channels in human retinal pigment epithelial (RPE) cells were investigated using the patch-clamp technique. Results: In cell-attached patches, an increase in BKCa-channel activity was induced by membrane stretch, which is mainly mediated by the increase in the number of long-lived openings. With inside-out patches, application of pipette suction did not modify single-channel conductance of BKCa channels; however, it did enhance the channel activity. Membrane stretch-induced channel activity was found to be independent of the level of intracellular Ca 2+. Membrane stretch also potentiated riluzole-stimulated BK Ca-channel activity. Conclusions: BKCa channel can be stimulated by membrane stretch in human RPE cells. During an increase in tensile strength of RPE, the BKCa channel might exert a protective action against deleterious tension by promoting a hyperpolarizing outflow that forces the cell to rest.",
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Stretch-stimulated activity of large conductance calcium-activated potassium channels in human retinal pigment epithelial cells. / Sheu, Shwu Jiuan; Wu, Sheng-Nan; Hu, Dan Ning.

In: Journal of Ocular Pharmacology and Therapeutics, Vol. 21, No. 6, 01.12.2005, p. 429-435.

Research output: Contribution to journalArticle

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