Structural basis of a flavivirus recognized by its neutralizing antibody: Solution structure of the domain III of the Japanese encephalitis virus envelope protein

Kuen Phon Wu, Chih Wei Wu, Ya Ping Tsao, Ting Wei Kuo, Yuan Chao Lou, Cheng Wen Lin, Suh Chin Wu, Jya Wei Cheng

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

The flavivirus envelope protein is the dominant antigen in eliciting neutralizing antibodies and plays an important role in inducing immunologic responses in the infected host. We have determined the solution structure of the major antigenic domain (domain III) of the Japanese encephalitis virus (JEV) envelope protein. The JEV domain III forms a β-barrel type structure composed of six antiparallel β-strands resembling the immunoglobulin constant domain. We have also identified epitopes of the JEV domain III to its neutralizing antibody by chemical shift perturbation measurements. Site-directed mutagenesis experiments are performed to confirm the NMR results. Our study provides a structural basis for understanding the mechanism of immunologic protection and for rational design of vaccines effective against flaviviruses.

Original languageEnglish
Pages (from-to)46007-46013
Number of pages7
JournalJournal of Biological Chemistry
Volume278
Issue number46
DOIs
Publication statusPublished - 2003 Nov 14

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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