TY - JOUR
T1 - Structures, Mechanisms, and Functions of His-Me Finger Nucleases
AU - Wu, Chyuan Chuan
AU - Lin, Jason L.J.
AU - Yuan, Hanna S.
N1 - Funding Information:
This work was supported by Academia Sinica ( AS-IA-105-L04 to H.S.Y.) and Ministry of Science and Technology (MOST 107-2321-B-001-035-MY3 to C.C.W.), Taiwan.
Publisher Copyright:
© 2020 The Author
PY - 2020/11
Y1 - 2020/11
N2 - His-Me finger (also called HNH or ββα-me) nucleases, are a large superfamily of nucleases that share limited sequence homology, but all members carry a highly similar catalytic motif exhibiting a ββα topology. This review represents a structural comparison of His-Me finger nucleases, summarizing their substrate-binding and recognition strategies, mechanisms of enzymatic hydrolysis, cellular functions, and the various means of activity regulation. His-Me finger nucleases usually function as monomers, making a single nick in nucleic acids to degrade foreign or host genomes, or as homodimers that introduce double-stranded DNA breaks for DNA restriction, integration, recombination, and repair. Various cellular neutralizing machineries have evolved to regulate the activity of His-Me finger nucleases, thereby maintaining genome integrity and cellular functionality.
AB - His-Me finger (also called HNH or ββα-me) nucleases, are a large superfamily of nucleases that share limited sequence homology, but all members carry a highly similar catalytic motif exhibiting a ββα topology. This review represents a structural comparison of His-Me finger nucleases, summarizing their substrate-binding and recognition strategies, mechanisms of enzymatic hydrolysis, cellular functions, and the various means of activity regulation. His-Me finger nucleases usually function as monomers, making a single nick in nucleic acids to degrade foreign or host genomes, or as homodimers that introduce double-stranded DNA breaks for DNA restriction, integration, recombination, and repair. Various cellular neutralizing machineries have evolved to regulate the activity of His-Me finger nucleases, thereby maintaining genome integrity and cellular functionality.
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U2 - 10.1016/j.tibs.2020.07.002
DO - 10.1016/j.tibs.2020.07.002
M3 - Review article
C2 - 32807610
AN - SCOPUS:85089501699
VL - 45
SP - 935
EP - 946
JO - Trends in Biochemical Sciences
JF - Trends in Biochemical Sciences
SN - 0376-5067
IS - 11
ER -