Structures, Mechanisms, and Functions of His-Me Finger Nucleases

Chyuan Chuan Wu, Jason L.J. Lin, Hanna S. Yuan

Research output: Contribution to journalReview articlepeer-review

6 Citations (Scopus)


His-Me finger (also called HNH or ββα-me) nucleases, are a large superfamily of nucleases that share limited sequence homology, but all members carry a highly similar catalytic motif exhibiting a ββα topology. This review represents a structural comparison of His-Me finger nucleases, summarizing their substrate-binding and recognition strategies, mechanisms of enzymatic hydrolysis, cellular functions, and the various means of activity regulation. His-Me finger nucleases usually function as monomers, making a single nick in nucleic acids to degrade foreign or host genomes, or as homodimers that introduce double-stranded DNA breaks for DNA restriction, integration, recombination, and repair. Various cellular neutralizing machineries have evolved to regulate the activity of His-Me finger nucleases, thereby maintaining genome integrity and cellular functionality.

Original languageEnglish
Pages (from-to)935-946
Number of pages12
JournalTrends in Biochemical Sciences
Issue number11
Publication statusPublished - 2020 Nov

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology


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