Study of cell transmigration using a co-culture microsystem under shear stress

C. H. Yeh; S. H. Tsai; Y. H. Huang; L. W. Wu; Y. C. Lin

doi:10.1016/j.proche.2009.07.090

Study of cell transmigration using a co-culture microsystem under shear stress

C. H. Yeh, S. H. Tsai, Y. H. Huang, L. W. Wu, Y. C. Lin

Research output: Contribution to journal › Conference article › peer-review

1 Citation (Scopus)

Abstract

This study reports a novel cell co-culture technique using micro-molding in capillaries (MIMIC) technology that was utilized to observe the transmigration conditions of two types of cells with and without fluidic shear stress. Besides, the gap size of co-culture device could achieve shortest and not mixture. Endothelial cells (ECs) and smooth muscle cells (SMCs) were used in our experiment. In addition, concentrations of two cell are 8000 cells/μL (ECs) and 9000 cells/μL (SMCs), respectively, the shear stress is 7 dyne/cm₂, and the isolation distance between two types of cell are 50 and 200 μm. It is found that in the smaller culture space (50 μm) condition, ECs and SMCs would induce mutually, which would further make cell migration; in larger culture space (200 μm) condition, no inducing reaction took place between ECs and SMCs. It will have more advantages in bio-manipulation and tissue repair engineering.

Original language	English
Pages (from-to)	361-364
Number of pages	4
Journal	Procedia Chemistry
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/j.proche.2009.07.090
Publication status	Published - 2009 Sept
Event	Eurosensors 23rd Conference - Lausanne, Switzerland Duration: 2009 Sept 6 → 2009 Sept 9

All Science Journal Classification (ASJC) codes

General Chemistry
General Chemical Engineering

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10.1016/j.proche.2009.07.090

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title = "Study of cell transmigration using a co-culture microsystem under shear stress",

abstract = "This study reports a novel cell co-culture technique using micro-molding in capillaries (MIMIC) technology that was utilized to observe the transmigration conditions of two types of cells with and without fluidic shear stress. Besides, the gap size of co-culture device could achieve shortest and not mixture. Endothelial cells (ECs) and smooth muscle cells (SMCs) were used in our experiment. In addition, concentrations of two cell are 8000 cells/μL (ECs) and 9000 cells/μL (SMCs), respectively, the shear stress is 7 dyne/cm2, and the isolation distance between two types of cell are 50 and 200 μm. It is found that in the smaller culture space (50 μm) condition, ECs and SMCs would induce mutually, which would further make cell migration; in larger culture space (200 μm) condition, no inducing reaction took place between ECs and SMCs. It will have more advantages in bio-manipulation and tissue repair engineering.",

author = "Yeh, {C. H.} and Tsai, {S. H.} and Huang, {Y. H.} and Wu, {L. W.} and Lin, {Y. C.}",

year = "2009",

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AU - Yeh, C. H.

AU - Tsai, S. H.

AU - Huang, Y. H.

AU - Wu, L. W.

AU - Lin, Y. C.

PY - 2009/9

Y1 - 2009/9

N2 - This study reports a novel cell co-culture technique using micro-molding in capillaries (MIMIC) technology that was utilized to observe the transmigration conditions of two types of cells with and without fluidic shear stress. Besides, the gap size of co-culture device could achieve shortest and not mixture. Endothelial cells (ECs) and smooth muscle cells (SMCs) were used in our experiment. In addition, concentrations of two cell are 8000 cells/μL (ECs) and 9000 cells/μL (SMCs), respectively, the shear stress is 7 dyne/cm2, and the isolation distance between two types of cell are 50 and 200 μm. It is found that in the smaller culture space (50 μm) condition, ECs and SMCs would induce mutually, which would further make cell migration; in larger culture space (200 μm) condition, no inducing reaction took place between ECs and SMCs. It will have more advantages in bio-manipulation and tissue repair engineering.

AB - This study reports a novel cell co-culture technique using micro-molding in capillaries (MIMIC) technology that was utilized to observe the transmigration conditions of two types of cells with and without fluidic shear stress. Besides, the gap size of co-culture device could achieve shortest and not mixture. Endothelial cells (ECs) and smooth muscle cells (SMCs) were used in our experiment. In addition, concentrations of two cell are 8000 cells/μL (ECs) and 9000 cells/μL (SMCs), respectively, the shear stress is 7 dyne/cm2, and the isolation distance between two types of cell are 50 and 200 μm. It is found that in the smaller culture space (50 μm) condition, ECs and SMCs would induce mutually, which would further make cell migration; in larger culture space (200 μm) condition, no inducing reaction took place between ECs and SMCs. It will have more advantages in bio-manipulation and tissue repair engineering.

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JF - Procedia Chemistry

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T2 - Eurosensors 23rd Conference

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ER -

Study of cell transmigration using a co-culture microsystem under shear stress

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