Study of cell transmigration using a co-culture microsystem under shear stress

C. H. Yeh, S. H. Tsai, Y. H. Huang, L. W. Wu, Y. C. Lin

Research output: Contribution to journalConference articlepeer-review

1 Citation (Scopus)


This study reports a novel cell co-culture technique using micro-molding in capillaries (MIMIC) technology that was utilized to observe the transmigration conditions of two types of cells with and without fluidic shear stress. Besides, the gap size of co-culture device could achieve shortest and not mixture. Endothelial cells (ECs) and smooth muscle cells (SMCs) were used in our experiment. In addition, concentrations of two cell are 8000 cells/μL (ECs) and 9000 cells/μL (SMCs), respectively, the shear stress is 7 dyne/cm2, and the isolation distance between two types of cell are 50 and 200 μm. It is found that in the smaller culture space (50 μm) condition, ECs and SMCs would induce mutually, which would further make cell migration; in larger culture space (200 μm) condition, no inducing reaction took place between ECs and SMCs. It will have more advantages in bio-manipulation and tissue repair engineering.

Original languageEnglish
Pages (from-to)361-364
Number of pages4
JournalProcedia Chemistry
Issue number1
Publication statusPublished - 2009 Sept
EventEurosensors 23rd Conference - Lausanne, Switzerland
Duration: 2009 Sept 62009 Sept 9

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • General Chemical Engineering


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