Study of cholesterol's effect on the properties of catanionic vesicular systems: Comparison of light-scattering results with ultrasonic and fluorescence spectroscopy

Martina Havlíková, Jana Szabová, Adam Jugl, Ludmila Mravcová, Chien Hsiang Chang, Po Sung Huang, Miloslav Pekař, Filip Mravec

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

This work is focused on the study of properties associated with the effect of cholesterol levels on the stability of vesicular systems based on the ion pair amphiphile hexadecyltrimethylammonium-dodecylsulphate (HTMA-DS) at laboratory temperature. The HTMA-DS catanionic system was doped with dioctadecyldimethylammonium chloride in a 9:1 M ratio and cholesterol in the amount of 0, 3, 13, 23, 33, 43, 53, 63, and 73 mol.% was added. In this system, the size distributions were studied using the dynamic light-scattering technique and the zeta potential was determined. These standard techniques were supplemented by ultrasonic and fluorescence spectroscopy techniques. Due to low stability and high opalescence of samples, spectral techniques were used only for the samples with cholesterol content above 23 mol.%. The results from High-Resolution Ultrasonic Spectroscopy and from Fluorescence Spectroscopy are in agreement. They equally point to a change in the amount of hydration water in the membrane, the largest amount of which is present in the samples with 43 and 53 mol.% cholesterol. Using the light-scattering technique, the short-term stability of prepared vesicular systems was also observed over the first 36 days. Obtained results confirmed that the most stable systems are those containing 43 or 53 mol.% of cholesterol.

Original languageEnglish
Article number125526
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume607
DOIs
Publication statusPublished - 2020 Dec 20

All Science Journal Classification (ASJC) codes

  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Fingerprint

Dive into the research topics of 'Study of cholesterol's effect on the properties of catanionic vesicular systems: Comparison of light-scattering results with ultrasonic and fluorescence spectroscopy'. Together they form a unique fingerprint.

Cite this