TY - GEN
T1 - Study on the gelsolin effects in oral epithelia carcinoma (OEC) cells by atomic force microscopy and micro-pillars arrays
AU - Chen, Wei Ren
AU - Huang, Pei Yu
AU - Wang, Chien Wen
AU - Lin, Yi Dong
AU - Lin, Chi Chang
AU - Chang, Hsien-Chang
PY - 2007/8/28
Y1 - 2007/8/28
N2 - According to the concept of cell physiology, when the cells contact the extracellular matrix (ECM), the cell will adhere, spread and migrates. Despite the sophistication of experimental and computational approaches in cell and molecular biology, the mechanisms by which cells sense and respond to mechanical stimuli are poorly understood. In order to evaluate the characteristics of the cellular physiology, we use Atomic force microscopy (AFM) and Micro-pillars arrays (MPAs) to measure the cell mechanics of the oral epithelia carcinoma cells (OEC-M1). After AFM measurement, the stiffness of the OEC-M1-wt was 41-95 kPa, meanwhile the OEC-M1-g6 was 21-46 kPa. In deed, 0.88-4.75 nN of the traction force was generated in OEC-M1-g6 cells, which the actin filament assemble around the cytoplasm might caused by the gelsolin, after forward, both the adherent and moving ability of the cells were increased.
AB - According to the concept of cell physiology, when the cells contact the extracellular matrix (ECM), the cell will adhere, spread and migrates. Despite the sophistication of experimental and computational approaches in cell and molecular biology, the mechanisms by which cells sense and respond to mechanical stimuli are poorly understood. In order to evaluate the characteristics of the cellular physiology, we use Atomic force microscopy (AFM) and Micro-pillars arrays (MPAs) to measure the cell mechanics of the oral epithelia carcinoma cells (OEC-M1). After AFM measurement, the stiffness of the OEC-M1-wt was 41-95 kPa, meanwhile the OEC-M1-g6 was 21-46 kPa. In deed, 0.88-4.75 nN of the traction force was generated in OEC-M1-g6 cells, which the actin filament assemble around the cytoplasm might caused by the gelsolin, after forward, both the adherent and moving ability of the cells were increased.
UR - http://www.scopus.com/inward/record.url?scp=34548126833&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548126833&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2007.352216
DO - 10.1109/NEMS.2007.352216
M3 - Conference contribution
AN - SCOPUS:34548126833
SN - 1424406102
SN - 9781424406104
T3 - Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
SP - 1129
EP - 1132
BT - Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
T2 - 2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
Y2 - 16 January 2007 through 19 January 2007
ER -