Substantial protective immunity conferred by a combination of Brucella abortus recombinant proteins against Brucella abortus 544 infection in BALB/c mice

Lauren Togonon Arayan, Tran Xuan Ngoc Huy, Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Vu Hai Son, Wongi Min, Hu Jang Lee, Suk Kim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Chronic infection with intracellular Brucella abortus (B. abortus) in livestock remains as a major problem worldwide. Thus, the search for an ideal vaccine is still ongoing. In this study, we evaluated the protective efficacy of a combination of B. abortus recombinant proteins; superoxide dismutase (rSodC), riboflavin synthase subunit beta (rRibH), nucleoside diphosphate kinase (rNdk), 50S ribosomal protein (rL7/L12) and malate dehydrogenase (rMDH), cloned and expressed into a pMal vector system and DH5α, respectively, and further purified and applied intraperitoneally into BALB/c mice. After first immunization and two boosters, mice were infected intraperitoneally (IP) with 5 × 10 4 CFU of virulent B. abortus 544. Spleens were harvested and bacterial loads were evaluated at two weeks post-infection. Results revealed that this combination showed significant reduction in bacterial colonization in the spleen with a log protection unit of 1.31, which is comparable to the average protection conferred by the widely used live attenuated vaccine RB51. Cytokine analysis exhibited enhancement of cell-mediated immune response as IFN-γ is significantly elevated while IL-10, which is considered beneficial to the pathogen’s survival, was reduced compared to control group. Furthermore, both titers of IgG1 and IgG2a were significantly elevated at three and four-week time points from first immunization. In summary, our in vivo data revealed that vaccination with a combination of five different proteins conferred a heightened host response to Brucella infection through cell-mediated immunity which is desirable in the control of intracellular pathogens. Thus, this combination might be considered for further improvement as a potential candidate vaccine against Brucella infection.

Original languageEnglish
Pages (from-to)330-338
Number of pages9
JournalJournal of Microbiology and Biotechnology
Volume29
Issue number2
DOIs
Publication statusPublished - 2019 Feb

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'Substantial protective immunity conferred by a combination of Brucella abortus recombinant proteins against Brucella abortus 544 infection in BALB/c mice'. Together they form a unique fingerprint.

Cite this