Subunit composition of ribosome in the yqgf mutant is deficient in pre-16S rRNA processing of Escherichia coli

Tatsuaki Kurata, Shinobu Nakanishi, Masayuki Hashimoto, Masato Taoka, Toshiaki Isobe, Jun Ichi Kato

Research output: Contribution to journalArticle

Abstract

Escherichia coli 16S, 23S, and 5S ribosomal RNAs (rRNAs) are transcribed as a single primary transcript, which is subsequently processed into mature rRNAs by several RNases. Three RNases (RNase III, RNase E, and RNase G) were reported to function in processing the 5′-leader of precursor 16S rRNA (pre-16S rRNA). Previously, we showed that a novel essential YqgF is involved in that processing. Here we investigated the ribosome subunits of the yqgF ts mutant by LC-MS/MS. The mutant ribosome had decreased copy numbers of ribosome protein S1, suggesting that the yqgF gene enables incorporation of ribosomal protein S1 into ribosome by processing of the 5′-end of pre-16S rRNA. The ribosome protein S1 is essential for translation in E. coli; therefore, our results suggest that YqgF converts the inactive form of newly synthesized ribosome into the active form at the final step of ribosome assembly.

Original languageEnglish
Pages (from-to)179-182
Number of pages4
JournalJournal of Molecular Microbiology and Biotechnology
Volume28
Issue number4
DOIs
Publication statusPublished - 2019 Jan 1

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Ribosome Subunits
RNA Precursors
Ribosomes
Escherichia coli
Ribonucleases
Ribonuclease III
16S Ribosomal RNA
Ribosomal RNA
Proteins
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Kurata, Tatsuaki ; Nakanishi, Shinobu ; Hashimoto, Masayuki ; Taoka, Masato ; Isobe, Toshiaki ; Kato, Jun Ichi. / Subunit composition of ribosome in the yqgf mutant is deficient in pre-16S rRNA processing of Escherichia coli. In: Journal of Molecular Microbiology and Biotechnology. 2019 ; Vol. 28, No. 4. pp. 179-182.
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Subunit composition of ribosome in the yqgf mutant is deficient in pre-16S rRNA processing of Escherichia coli. / Kurata, Tatsuaki; Nakanishi, Shinobu; Hashimoto, Masayuki; Taoka, Masato; Isobe, Toshiaki; Kato, Jun Ichi.

In: Journal of Molecular Microbiology and Biotechnology, Vol. 28, No. 4, 01.01.2019, p. 179-182.

Research output: Contribution to journalArticle

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