TY - JOUR
T1 - Subunit composition of ribosome in the yqgf mutant is deficient in pre-16S rRNA processing of Escherichia coli
AU - Kurata, Tatsuaki
AU - Nakanishi, Shinobu
AU - Hashimoto, Masayuki
AU - Taoka, Masato
AU - Isobe, Toshiaki
AU - Kato, Jun Ichi
N1 - Funding Information:
We thank Dr. Katsumi Isono for critical reading of the manuscript and much advice. We also thank C. Fujita for support of this study. This work was supported by KAKENHI from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
Publisher Copyright:
© 2018 S. Karger AG, Basel.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Escherichia coli 16S, 23S, and 5S ribosomal RNAs (rRNAs) are transcribed as a single primary transcript, which is subsequently processed into mature rRNAs by several RNases. Three RNases (RNase III, RNase E, and RNase G) were reported to function in processing the 5′-leader of precursor 16S rRNA (pre-16S rRNA). Previously, we showed that a novel essential YqgF is involved in that processing. Here we investigated the ribosome subunits of the yqgF ts mutant by LC-MS/MS. The mutant ribosome had decreased copy numbers of ribosome protein S1, suggesting that the yqgF gene enables incorporation of ribosomal protein S1 into ribosome by processing of the 5′-end of pre-16S rRNA. The ribosome protein S1 is essential for translation in E. coli; therefore, our results suggest that YqgF converts the inactive form of newly synthesized ribosome into the active form at the final step of ribosome assembly.
AB - Escherichia coli 16S, 23S, and 5S ribosomal RNAs (rRNAs) are transcribed as a single primary transcript, which is subsequently processed into mature rRNAs by several RNases. Three RNases (RNase III, RNase E, and RNase G) were reported to function in processing the 5′-leader of precursor 16S rRNA (pre-16S rRNA). Previously, we showed that a novel essential YqgF is involved in that processing. Here we investigated the ribosome subunits of the yqgF ts mutant by LC-MS/MS. The mutant ribosome had decreased copy numbers of ribosome protein S1, suggesting that the yqgF gene enables incorporation of ribosomal protein S1 into ribosome by processing of the 5′-end of pre-16S rRNA. The ribosome protein S1 is essential for translation in E. coli; therefore, our results suggest that YqgF converts the inactive form of newly synthesized ribosome into the active form at the final step of ribosome assembly.
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U2 - 10.1159/000494494
DO - 10.1159/000494494
M3 - Article
C2 - 30566952
AN - SCOPUS:85059197741
VL - 28
SP - 179
EP - 182
JO - Journal of Molecular Microbiology and Biotechnology
JF - Journal of Molecular Microbiology and Biotechnology
SN - 1464-1801
IS - 4
ER -