Abstract
Escherichia coli 16S, 23S, and 5S ribosomal RNAs (rRNAs) are transcribed as a single primary transcript, which is subsequently processed into mature rRNAs by several RNases. Three RNases (RNase III, RNase E, and RNase G) were reported to function in processing the 5′-leader of precursor 16S rRNA (pre-16S rRNA). Previously, we showed that a novel essential YqgF is involved in that processing. Here we investigated the ribosome subunits of the yqgF ts mutant by LC-MS/MS. The mutant ribosome had decreased copy numbers of ribosome protein S1, suggesting that the yqgF gene enables incorporation of ribosomal protein S1 into ribosome by processing of the 5′-end of pre-16S rRNA. The ribosome protein S1 is essential for translation in E. coli; therefore, our results suggest that YqgF converts the inactive form of newly synthesized ribosome into the active form at the final step of ribosome assembly.
| Original language | English |
|---|---|
| Pages (from-to) | 179-182 |
| Number of pages | 4 |
| Journal | Journal of Molecular Microbiology and Biotechnology |
| Volume | 28 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2019 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Microbiology
- Applied Microbiology and Biotechnology
- Molecular Biology
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Subunit composition of ribosome in the yqgf mutant is deficient in pre-16S rRNA processing of Escherichia coli. / Kurata, Tatsuaki; Nakanishi, Shinobu; Hashimoto, Masayuki; Taoka, Masato; Isobe, Toshiaki; Kato, Jun Ichi.
In: Journal of Molecular Microbiology and Biotechnology, Vol. 28, No. 4, 01.01.2019, p. 179-182.Research output: Contribution to journal › Article
TY - JOUR
T1 - Subunit composition of ribosome in the yqgf mutant is deficient in pre-16S rRNA processing of Escherichia coli
AU - Kurata, Tatsuaki
AU - Nakanishi, Shinobu
AU - Hashimoto, Masayuki
AU - Taoka, Masato
AU - Isobe, Toshiaki
AU - Kato, Jun Ichi
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Escherichia coli 16S, 23S, and 5S ribosomal RNAs (rRNAs) are transcribed as a single primary transcript, which is subsequently processed into mature rRNAs by several RNases. Three RNases (RNase III, RNase E, and RNase G) were reported to function in processing the 5′-leader of precursor 16S rRNA (pre-16S rRNA). Previously, we showed that a novel essential YqgF is involved in that processing. Here we investigated the ribosome subunits of the yqgF ts mutant by LC-MS/MS. The mutant ribosome had decreased copy numbers of ribosome protein S1, suggesting that the yqgF gene enables incorporation of ribosomal protein S1 into ribosome by processing of the 5′-end of pre-16S rRNA. The ribosome protein S1 is essential for translation in E. coli; therefore, our results suggest that YqgF converts the inactive form of newly synthesized ribosome into the active form at the final step of ribosome assembly.
AB - Escherichia coli 16S, 23S, and 5S ribosomal RNAs (rRNAs) are transcribed as a single primary transcript, which is subsequently processed into mature rRNAs by several RNases. Three RNases (RNase III, RNase E, and RNase G) were reported to function in processing the 5′-leader of precursor 16S rRNA (pre-16S rRNA). Previously, we showed that a novel essential YqgF is involved in that processing. Here we investigated the ribosome subunits of the yqgF ts mutant by LC-MS/MS. The mutant ribosome had decreased copy numbers of ribosome protein S1, suggesting that the yqgF gene enables incorporation of ribosomal protein S1 into ribosome by processing of the 5′-end of pre-16S rRNA. The ribosome protein S1 is essential for translation in E. coli; therefore, our results suggest that YqgF converts the inactive form of newly synthesized ribosome into the active form at the final step of ribosome assembly.
UR - http://www.scopus.com/inward/record.url?scp=85059197741&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85059197741&partnerID=8YFLogxK
U2 - 10.1159/000494494
DO - 10.1159/000494494
M3 - Article
C2 - 30566952
AN - SCOPUS:85059197741
VL - 28
SP - 179
EP - 182
JO - Journal of Molecular Microbiology and Biotechnology
JF - Journal of Molecular Microbiology and Biotechnology
SN - 1464-1801
IS - 4
ER -