Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair

William J. Feaver, Wen-Ya Huang, Opher Gileadi, Lawrence Myers, Claes M. Gustafsson, Roger D. Kornberg, Errol C. Friedberg

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A yeast strain harboring a temperature-sensitive allele of TFB3 (tfb3(ts)), the 38-kDa subunit of the RNA polymerase II transcription/nucleotide excision repair factor TFIIH, was found to be sensitive to ultraviolet (UV) radiation and defective for nucleotide excision repair in vitro. Interestingly, tfb3(ts) failed to grow on medium containing caffeine. A comprehensive pairwise two-hybrid analysis between yeast TFIIH subunits identified novel interactions between Rad3 and Tfb3, Tfb4 and Ssl1, as well as Ssl2 and Tfb2. These interactions have facilitated a more complete model of the structure of TFIIH and the nucleotide excision repairosome.

Original languageEnglish
Pages (from-to)5941-5946
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number8
DOIs
Publication statusPublished - 2000 Feb 25

Fingerprint

Transcription Factor TFIIH
Transcription
DNA Repair
Yeast
Repair
Nucleotides
Yeasts
RNA Polymerase II
Caffeine
Alleles
Radiation
Ultraviolet radiation
Temperature

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Feaver, William J. ; Huang, Wen-Ya ; Gileadi, Opher ; Myers, Lawrence ; Gustafsson, Claes M. ; Kornberg, Roger D. ; Friedberg, Errol C. / Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 8. pp. 5941-5946.
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Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair. / Feaver, William J.; Huang, Wen-Ya; Gileadi, Opher; Myers, Lawrence; Gustafsson, Claes M.; Kornberg, Roger D.; Friedberg, Errol C.

In: Journal of Biological Chemistry, Vol. 275, No. 8, 25.02.2000, p. 5941-5946.

Research output: Contribution to journalArticle

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