Suction-type microfluidic immunosensing systems for rapid detection of dengue fever

C. H. Weng, T. B. Huang, C. C. Huang, C. S. Yeh, H. Y. Lei, G. B. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study reports an integrated microfluidic system for rapid immunosensing of antibodies associated with dengue fever by utilizing enzyme-linked immunoabsorbent assay (ELISA) poly(dimethylsiloxane) (PDMS)-based chip. In this study, we successfully developed a rapid immunosensing chip with pre-functionalized surfaces to avoid protein adsorption. The functionalized PDMS surface was found to be highly effective in reducing the adsorption time in microchannels. The functionalized surfaces can be stable at least for 1 month when stored at 4°C. In addition, a suction-type microfluidic transportation module was used to form a single-fluid-slug transportation and to mix the reagents for rapid detection of dengue virus. The entire process can be finished within 30 min. The sensitivity was found to be superior to the conventional methods.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages97-99
Number of pages3
Publication statusPublished - 2010 Dec 1
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume1

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10-10-0310-10-07

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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